Network Facilities and Characterisation Techniques:

The instrumentation available in the various nodes of the Network are:

Major Research Techniques

  • Spectroscopy
    • Multi-Colour and White Light Pump-probe transient absorption (femtoseconds to seconds)
    • Multi-Colour Spectrally Resolved Three-Pulse Photon Echo Peak Shift
    • Femtosecond Kerr-gated fluorescence
    • Femtosecond fluorescence upconversion
    • Stimulated emission decay measurements
    • Picosecond fluorescence measurements by Time-Correlated Single Photon Counting (TCSPC)
  • Microscopy capabilities
    • Single- and Multi-Photon Laser Scanning Confocal time-resolved fluorescence imaging
    • Structured Illumination Microscopy (SIM)
    • Scanning Near Field Optical Microscopy (SNOM)
    • Stimulated Emission Depletion Microscopy (STED)
    • Time Resolved Total Internal Reflection Fluorescence Microscopy (TIRFM) – Evanescent Wave Induced Fluorescence for FLIM and SLIM
    • Single Molecule Fluorescence Correlation Spectroscopy
  • Time-Resolved Fluorescence Spectroscopy and Anisotropy (Bulk)

Equipment

  • Femtosecond (Coherent Amplified System)
    • A mode-locked titanium sapphire laser (Mira-Seed: 20 fs, 76 MHz, 0.5 W)
    • Biophotonics Solutions MIIPS 640P pulse shaper
    • A chirped-pulse regenerative-amplifier (RegA9050: ~50 fs, 10-250 kHz, 0.1-2.5 W)
    • Two optical parametric amplifiers (OPA9050: ~50 fs, [>nJ] 400 nm, [~0.15 nJ] 480-700 nm, [~20 pJ] 900-2300 nm)
  • Femtosecond (High-Repetition Rate)
    • A cavity-dumped mode-locked titanium sapphire laser (Mira 900/PulseSwitch: ~120 fs, 76 MHz and 54 MHz to 200 Hz, 710-900 nm, ~40 nJ)
    • Doubler and Tripler (240-300 nm, 350-450 nm)
    • Optical Parametric Oscillator (APE: intracavity doubled)
  • Femtosecond (High-Repetition Rate)
    • KML Ti:sapp. oscillator (15 fs @ 80 MHz)
  • Picosecond
    • Fianium SC450-PP-HE super-continuum laser
    • NKT super-continuum laser/Varia
    • Time-Bandwidth Products Cheetah X, frequency doubled picosecond Nd:Vanadate laser
    • Two cavity-dumped dye lasers (Spectra Physics)
  • Nanosecond
    • Q-Switched Nd:YAG (Continuum NY-61)
    • Optical parametric oscillator (Casix)
  • Microscopes
    • Laser Scanning Confocal (Olympus IX71/FV300)
    • TIRF (Nikon TE2000)
    • Structured Illumination Microscopy
    • Scanning near field optical microscopy (SNOM) (Nanonics)
    • STED microscopy
    • Single Molecule
    • EMCCD (Roper)
    • sCMOS (Andor)
  • Time-resolved microscopy techniques
    • TCSPC (Becker and Hickl SPC830 & SPC150, Edinburgh Instruments TCC900 cards)
    • Gated photon counting (Nikon LIMO)
    • High repetition rate intensified CCD camera (La Vision PicoStarHR)
  • Misc
    • Liquid nitrogen/helium cryostats for low temperature measurements using any of the above techniques
    • Cary UV-Vis and Fluorescence spectrometers
    • Reflectance spectrometer
    • Quartz Crystal Microbalance