Our lab undertakes research on adaptation of organisms (particularly invertebrates) to environmental stresses including climate change and chemical pollutants, using field sites in the Victorian mountains, in tropical rainforests and in wetlands around Melbourne. We also develop integrated pest control options, investigate how landscape changes can be harnessed to provide pest control services, contribute to novel approaches for suppressing dengue mosquito vectors, and examine new ways to predict species distribution shifts under climate change.
We focuses on three main areas of research:
- Climatic Stress – to find traits that enable adaptation to climatic stress, and to understand the genetic basis of these traits;
- Applied – to develop better ways of sustainably controlling pest species important from an agricultural perspective.
- Applied – to explore new ways of reducing the transmission of human diseases by insect vectors based on endosymbionts.
In understanding the main issues and mechanisms involved with adaptation of organisms to stress, we are able to make informed management decisions to assist the community and industry.
Molecular population markers, ecological assessments, pesticide assays, quantitative genetic analyses, molecular species markers and entomological sampling.
The economic benefit of biodiversity in agriculture
Words: Linda Thomson Image: Zagrammosoma latilineatum by Elia Pirtle Along with sustainability, biodiversity is a current catchword. Our work demonstrating the benefits of non crop vegetation on increasing biodiversity and especially "beneficials' which contribute to pest control in crops. Enthusiasm for the project is shown by the excellent attendance at a recent workshop – report of which has been highlighted as an …7 September, 2018 Agriculture, pe...
Progressing genetic rescue with eastern barred bandicoots
Words: Ary Hoffmann Cover image: John Gould 1863 As featured recently in a Pursuit piece, we are making steady progress with the genetic rescue of eastern barred bandicoots through our joint work with Mt Rothwell sanctuary. Genetic rescue provides a way of introducing new genetic material into threatened populations which in turn allows these populations to increase their fitness and ability to …6 September, 2018 threatened spec...
A new and unusual Wolbachia bacteria from Drosophila flies limited to the female sex
Words: Ary Hoffmann Cover image: Perran Ross As Wolbachia bacteria that live inside insect cells continue to be discovered and studied in detail, our appreciation of the diverse ways in which these bacteria interact with their hosts continues to expand. In past work we have found Wolbachia that cause embryo death when infected males mate with uninfected females (“cytoplasmic incompatibility”), Wolbachia that …24 August, 2018 wolbachia, endo...
Probing the void for blood
Words and video: Perran Ross I recently filmed one of our mosquito colonies trying desperately to reach my arm through their enclosure. The video has been posted on Reddit by a third party and received enormous attention with over 4.5 million views in its first nine hours. To make the video, I held my arm next to a cage of Aedes aegypti …10 July, 2018 mosquitoes, wol...
We have a logo!
Excellent design by Elia Pirtle, as you can see our beleaguered website manager hasn't quite figured out how to make it work as a banner image on this site.27 June, 2018 News
New paper | Interspecific hybridization may provide novel opportunities for coral reef restoration
A new paper is out in Frontiers in Marine Science - article link A nicely digestible review of the article is available at Ocean bites here27 June, 2018 conservation, c...
Large male mosquitoes unluckier in love
Words and images: Perran Ross Large male mosquitoes may have more trouble than smaller males in finding a partner. In a new study, we find that small female mosquitoes tend to avoid larger males, preferring to mate with smaller ones. In this study, now available as a pre-print on bioRxiv, we performed laboratory experiments to investigate the effect of male and …31 May, 2018 mosquitoes, Aed...
New publication | Fine-scale landscape genomics helps explain the slow spatial spread of Wolbachia through the Aedes aegypti population in Cairns, Australia
Author summary and figures by Tom Schmidt Wolbachia is a bacterium that suppresses the capacity for arbovirus transmission in the mosquito Aedes aegypti, and can spread spatially through wild mosquito populations following local introductions. Recent introductions in Cairns, Australia have successfully established Wolbachia in the Ae. aegypti population, but the infection has spread more slowly than expected through the surrounding area. Potential reasons …25 May, 2018 wolbachia, Aede...
Insect Armageddon!!? | Ary and others discuss on ABC Radio National Science Friction
Header photo by Museums Victoria, CC BY. Photographer: Rodney Start Original broadcast Sunday 20 May 2018 http://www.abc.net.au/radionational/programs/sciencefriction/insect-armageddon/977565421 May, 2018 News
NEW PROJECT | Buruli ulcer
Words and photo: Jason Axford On 26 April at the Peter Doherty Institute, Federal Minister for Health, Greg Hunt, announced new NHMRC funding to investigate the mysterious and rather horrific disease commonly known as Buruli ulcer (BU) (formerly known as Bairnsdale ulcer). The project is led by Prof. Tim Stinear in the Department of Microbiology and Immunology at the Peter Doherty Institute …10 May, 2018 mosquitoes, pes...
NEW PUBLICATION | The influence of host adaptation on crop rotations in managing the redlegged earth mite
Words and photo: Xuan Cheng This research has been published in the Journal of Economic Entomology The redlegged earth mite, Halotydeus destructor (Tucker) (Trombidiformes: Penthaleidae) is a polyphagous economic pest in Australia, attacking a broad range of host plants in broadacre farming systems1. Although applications of pesticides are currently the main method in managing earth mites, resistance against pyrethroid and organophosphate chemicals …10 May, 2018 News
Biology of the babushkas
Words: Samantha Ward As the name suggests, at the Pest & Environmental Adaptation Research Group we are interested in pest species and enjoy investigating novel approaches to sustainably control such organisms. I began my PhD at PEARG in 2016 studying parasitoid wasps; A group of wasps that lay their eggs inside or onto other arthropods. Parasitoids differ from parasites because they …10 May, 2018 Agriculture, pe...
Ambiguous literature | kdr triple resistance mutation – Where has it really been found?
Editors note: This article is direct from our internal research diary Words: Nancy M. Endersby-Harshman The purpose of this article is to recommend very careful reading and analysis of the literature relating to sodium channel mutations in Aedes aegypti and equal care in writing about them. I have uncovered some confusion in the mosquito literature about the kdr triple resistance mutation in the …4 May, 2018 mosquitoes, Aed...
PEARG on the air tonight
Tune in to 3RRR 102.7 FM between 7-8 this evening to hear Ary in discussion about all things insect ecology. If you miss it or live too far from Melbourne to tune in, you can stream the show live at rrr.org.au or after it airs at https://www.rrr.org.au/program/greening-the-apocalypse/ 3RRR is a Melbourne based community radio station, this show in particular is usually quite …1 May, 2018 News, Blog
Rapid evolution in insect pest species
Off the back of a review Ary wrote last year in Current Opinion in Insect Science, a less technical and more digestible summary is now available (sans pay-wall) at Science Trends. A Quote from the Science Trends article to grab your interest: Evolutionary changes often occur when pest species invade new areas where they have not been found before ... Pests are …30 April, 2018 Agriculture, pe...
Collecting fresh mosquitoes | PEARG in the field
Words: Tom Schmidt Photos: Tom Schmidt and Perran Ross At PEARG, we have a great interest in environmental pests and how to deal with them. One of these pests is the mosquito Aedes aegypti, which causes catastrophic damage throughout the world’s tropical zone. Aedes aegypti spreads viruses such as dengue, Zika and chikungunya, and is highly adept at invading new regions around …30 April, 2018 mosquitoes, wol...
The Wolbachia pandemic | Symbionts spread rapidly across highly diverged flies
Words: Perran Ross Cover photo: Andrew Weeks Wolbachia are perhaps the most prevalent bacterial symbionts on earth. Of the millions of insect species, Wolbachia are estimated to infect up to half of them. These bacteria are renowned for the effects they exert on their hosts, which can often be quite dramatic. Some Wolbachia strains are highly pathogenic; for instance, the wMelPop, or …16 April, 2018 wolbachia, endo...
WOLBACHIA BACTERIA IN ACTION | How we’re using naturally occurring bacteria to stop mosquitoes from spreading disease
Words and images: Perran Ross Cover photo: Jason Axford Dengue is a major global health issue. It infects millions of people every year and can cause debilitating illness, inflicting joint pain, rash and fever. Without any effective vaccine, the best way to prevent dengue is to target the mosquitoes that transmit it. Dengue is spread by Aedes mosquitoes, and we’ve been waging …13 April, 2018 mosquitoes, wol...
New review | The detection and significance of emerging insecticide resistance in mosquitoes
Nancy, Andrew and Ary have a fresh review article in CSIRO's 'Microbiology Australia' journal. Below is the abstract, for the full text please follow this link. Mosquito-borne arboviruses are increasing in incidence around the world. Australia enjoys some protection from pests and diseases afforded by its geographic isolation coupled with strict biosecurity control at its borders. However, as the volume of …11 April, 2018 mosquitoes, wol...
What’s in a (species) name? Using genetics to map the hidden diversity of earwigs
Written by Oliver Stuart, Image credit: Ryan Hodnett [CC BY-SA 4.0 (https://creativecommons.org/licenses/by-sa/4.0)], from Wikimedia Commons There are a lot of different insects; this cannot be overstated. Of the roughly 2 million animal species (that we know of), insects make up well over half. This mega-diversity has been a source of delight for biologists for centuries, but it has also caused no …11 April, 2018 Agriculture, ta...
Scientific Advisory Committee supports recommendation Keyacris scurra for listing under the Flora and Fauna Guarantee Act
Progress! For those unfamiliar, the Flora and Fauna Guarantee Act 1988 is "the key piece of Victorian legislation for the conservation of threatened species and communities and for the management of potentially threatening processes.", you can find more information onthe act and it's significance for protecting threatened species in Victoria at the DELWP website. You can read the full Scientific Advisory Committee's …14 March, 2018 threatened spec...
Study Shows Rapid Evolution in Lab-Reared Insects | Perran and Ary’s new paper covered in Entomology Today
Perran and Ary's lab adaptation review paper is fresh off the press and immediately generating interest. Follow the link below to Entomology Today to read all about it. https://entomologytoday.org/2018/03/01/study-shows-rapid-evolution-in-lab-reared-insects/2 March, 2018 mosquitoes, Aed...
Picky feeders: Mosquitoes turn their nose up at non-human blood
Words: Ellen Cottingham Image: Perran Ross Most Australians have sat outside on a summers evening only to return inside to find their legs and arms dotted with fresh itchy mozzie bites. It is not widely known that only female mosquitoes bite warm blooded animals or humans. The reason for this is that females use protein and iron from the blood to help …23 February, 2018 mosquitoes, wol...
Climate contributes to the evolution of pesticide resistance | James Maino, Paul Umina, Ary Hoffmann AbstractThe evolution of pesticide resistance through space and time is of great economic significance to modern agricultural production systems, and consequently, is often well documented. It can thus be used to dissect the evolutionary and ecological processes that underpin large-scale evolutionary responses. There are now …19 February, 2018 mosquitoes, wol...
Keys matchstick grasshopper is disappearing | ABC Radio interview #2
Mike Kearney was interviewed on The ABC's Victorian Country hour on Friday. Link below, interview begins 38:40. Victorian Country Hour for Friday 16th February 201819 February, 2018 threatened spec...
Grasshopper on the brink of extinction in Victoria | ABC Radio interview
Ary was interviewed on ABC Radio Melbourne on yesterdays afternoon program with Richelle Hunt. Great to see some public interest in the conservation of an invertebrate! Link9 February, 2018 threatened spec...
Grasshoppers: The new poster bug for insect conservation
Ary and Michael Kearney have written a new article in Pursuit on the conservation of Key’s matchstick grasshopper (Keyacris scurra). The story has been picked up by major newspapers The Age | Sydney Morning Herald Watch this space for more grasshopper news. Featured image credit: Michael Kearney8 February, 2018 threatened spec...
Ary featured in GRDC’s GroundCover™ (South) issue 132: January – February 2018
Originally published at https://grdc.com.au/resources-and-publications/groundcover/groundcover-132-january-february-20187 February, 2018 Agriculture, Ne...
We believe research should be open and accessible to all wherever possible.
This is an ongoing project, please check back for new additions as we compile and share our datasets.
My focus is on pest control & environmental stress adaptation. Undertaking research on invertebrates, developing integrated pest control options for the grains industries, investigating how landscape changes can be harnessed to provide pest control services & contributing to novel approaches for suppressing dengue mosquito vectors. We focus on adaptation to rapid environmental changes in a range of species, developing ways of incorporating evolutionary approaches when making predictions about adaptive capacity & when predicting species distribution shifts under climate change. We have expertise in genetics and genomics, pest entomology & physiological ecology.
Perran has been a member of the Hoffmann lab since 2012, where he previously completed a MSc and PhD working with Aedes aegypti mosquitoes. He is interested in disease control programs that deploy modified insects into natural populations and the factors that could affect their success. During his PhD, he investigated the effects of different environmental conditions on Wolbachia infections and the mosquitoes they infect. His current research will involve generating new Wolbachia infections in Aedes aegypti and insect pests to provide more options for pest and disease vector control.
- Generating new Wolbachia infections for pest and disease vector control
- Laboratory adaptation and inbreeding in mosquitoes
- Wolbachia infections under heat stress
- Ross, P. A., Axford, J. K., Richardson, K. M., Endersby-Harshman, N. M., & Hoffmann, A. A. (2017). Maintaining Aedes aegypti mosquitoes infected with Wolbachia. Journal of visualized experiments. In press.
- Ross, P. A., Wiwatanaratanabutr, I., Axford, J. K., White, V. L., Endersby-Harshman, N. M., & Hoffmann, A. A. (2017). Wolbachia infections in Aedes aegypti differ markedly in their response to cyclical heat stress. PLoS pathogens, 13(1), e1006006.
- Ross, P. A., Endersby, N. M., & Hoffmann, A. A. (2016). Costs of three Wolbachia infections on the survival of Aedes aegypti larvae under starvation conditions. PLoS neglected tropical diseases, 10(1).
- Axford, J. K., Ross, P. A., Yeap, H. L., Callahan, A. G., & Hoffmann, A. A. (2015). Fitness of wAlbB Wolbachia infection in Aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion. The American journal of tropical medicine and hygiene, 15-0608.
- Hoffmann, A. A., Ross, P. A., & Rašić, G. (2015). Wolbachia strains for disease control: ecological and evolutionary considerations. Evolutionary applications 8(8), 751-768.
- Ross, P. A., Endersby, N. M., Yeap, H. L., & Hoffmann, A. A. (2014). Larval competition extends developmental time and decreases adult size of wMelPop Wolbachia-infected Aedes aegypti. The American journal of tropical medicine and hygiene, 91(1), 198-205.
Genetics Society of Australasia 2014 “Larval competition reduces the fitness of Wolbachia-infected Aedes aegypti”
American Society of Tropical Medicine and Hygiene 2015 “Wolbachia infection reduces the starvation resistance of Aedes aegypti larvae”
Mosquito Control Association of Australia 2016 “Wolbachia infections in Aedes aegypti are adversely affected by cyclical heat stress”
Australian Fly Meeting 2016 “Environmental impacts on the use of Wolbachia for arbovirus control”
IMPACT7 2017 “Using bacteria to fight mosquito-borne disease”
Royal Society of Victoria Young Scientist Research Prize (Finalist)
Australian Government Research Training Program Scholarship
School of BioSciences Travel Scholarship
Bio21 Travel Scholarship
Personal website: perranross.wordpress.com
Google Scholar: goo.gl/WZH0Jp
Nancy Endersby-Harshman, Research Fellow and Laboratory manager
Nancy is a Research Fellow in the Pest and Environmental Adaptation Research Group in the Hoffmann Laboratory, Bio21 Institute, School of BioSciences at the University of Melbourne. Nancy has a background in horticultural entomology and a PhD from Monash University on the genetic structure of Australian populations of the horticultural pest, the diamondback moth, Plutella xylostella. Nancy currently conducts research on the dengue vector mosquito, Aedes aegypti, in the fields of insecticide resistance, population genetics, Wolbachia pipientis and vector ecology. Nancy manages the group’s Molecular Laboratory and the Arboviral Research Laboratory, a Biosecurity Approved Arrangement with the Department of Agriculture and Water Resources.
Our laboratory studies release of Wolbachia-infected mosquitoes (Aedes aegypti) to reduce the impact of dengue virus. Wolbachia-infected mosquitoes do not transmit dengue and we aim to replace the natural population of the dengue vector with the same mosquito species in which virus replication is blocked. The Wolbachia strategy relies on the released mosquitoes being fit and competitive in the field and different strains of the Wolbachia bacterium may have differing fitness costs to the mosquito. So, an important part of our research is to do with maintaining a high level of quality in our mosquitoes for release.
After Wolbachia-infected mosquitoes were released with success in Cairns, the program was mobilised with releases occurring in parts of Asia and South America where different levels of success in establishment of the Wolbachia mosquito lines have been experienced. Consequently, we now investigate environmental factors which may act as barriers to establishment of Wolbachia strains in the environment in an effort to find strains that will perform robustly in the field.
Current projects in this field look at
- effect of environmental levels of antibiotic contamination on Wolbachia-infected mosquitoes
- background levels of insecticide resistance in Aedes aegypti at potential release sites with a particular focus on pyrethroid insecticides and target site resistance
- Other insecticide resistance projects include assessment of resistance status of Ae. aegypti which arrive in Australia as exotic incursions in comparison with the local population of Ae. aegypti mosquitoes in Queensland.
Outbreaks of dengue fever have occurred in Queensland, Australia, since the late 1800s, leading to ongoing attempts to control the mosquito vector, Aedes aegypti (L.). Since the 1990s, pyrethroid insecticides have been used for this purpose, but have been applied in a strategic manner with a variety of delivery methods including indoor residual spraying, lethal ovitraps and use of insect growth regulators as larvicides. Separate selection experiments that we conducted on mosquitoes from Queensland, using Type I and Type II pyrethroids did not produce resistant lines of Ae. aegypti and bioassays of field material from Queensland showed only weak tolerance in comparison with a susceptible line. There was no evidence of knockdown resistance (kdr) mutations in Ae. aegypti from Queensland, in stark contrast to the situation in nearby southeast Asia. We suspect that careful management of pyrethroid insecticide use combined with surveillance and interception of exotic incursions has helped to maintain pyrethroid (and particularly kdr – based) susceptibility in Ae. aegypti in Australia.
Refereed Journals (first, joint first or senior author)
Endersby-Harshman, NM, Wuliandari JR, Harshman LG, Frohn V, Johnson BJ, Ritchie SA, Hoffmann AA. 2017. Pyrethroid susceptibility has been maintained in the dengue vector, Aedes aegypti (Diptera: Culicidae), in Queensland, Australia. Journal of Medical Entomology 54: 1649-1658.
Rašić G*, Endersby-Harshman NM*, Tantowijoyo W, Goundar A, White VL, Yang Q, Filipović I, Johnson P, Hoffmann AA, Arguni E (2015) Aedes aegypti has spatially structured and seasonally stable populations in Yogyakarta, Indonesia. Parasites & Vectors 12/2015; 8(1). DOI:10.1186/s13071-015-1230-6 *Joint first authors.
Rochmijati Wuliandari J, Lee SF, White VL, Tantowijoyo W, Hoffmann AA, Endersby-Harshman NM (2015). Association between three mutations, F1565C, V1023G and S996P, in the voltage-sensitive sodium channel gene and knockdown resistance in Aedes aegypti from Yogyakarta, Indonesia. Wang C, ed. Insects. 2015; 6 (3): 658-685. doi:10.3390/insects6030658.
Endersby NM, White VL, Chan J, Hurst T, Rašić G, Miller A, Hoffmann AA (2013). Evidence of cryptic genetic lineages within Aedes notoscriptus (Skuse). Infection, Genetics and Evolution 18: 191-201.
Endersby NM & Hoffmann AA (2013). Effect of Wolbachia on insecticide susceptibility in lines of Aedes aegypti. Bulletin of Entomological Research 103 (3), 269-277.
Endersby NM, Hoffmann AA, White VL, Ritchie S, Johnson PH, Rapley LP, Weeks AR (2011). Changes in the genetic structure of Aedes aegypti (Diptera: Culicidae) populations in Queensland, Australia, across two seasons: Implications for potential mosquito releases. Journal of Medical Entomology 48 (5): 999-1007.
Endersby NM, Viduka K, Baxter SW, Saw J, Heckel DG & McKechnie SW (2011). Widespread pyrethroid resistance in Australian diamondback moth, Plutella xylostella (L.), is related to multiple mutations in the para sodium channel gene. Bulletin of Entomological Research 101: 393–405.
Endersby NM, Hoffmann AA, White VL, Lowenstein S, Ritchie S, Johnson PH, Rapley LP, Ryan PA, Nam VS, Yen NT, Kittiyapong P, Weeks AR (2009). Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and Exon Primed Intron Crossing markers suggests feasibility of local control options. Journal of Medical Entomology 46 (5): 1074-1083.
Endersby NM, Ridland PM and Hoffmann AA (2008). The effects of local selection versus dispersal on insecticide resistance patterns: longitudinal evidence from diamondback moth (Plutella xylostella (Lepidoptera: Plutellidae)) in Australia evolving resistance to pyrethroids. Bulletin of Entomological Research 98: 145 – 157.
Endersby NM, Hoffmann AA, McKechnie SW and Weeks AR (2007). Is there genetic structure in populations of Helicoverpa armigera in Australia? Entomologia Experimentalis et Applicata 122: 253-263.
Endersby NM, McKechnie SW, Ridland PM and Weeks AR (2006). Microsatellites reveal a lack of structure in Australian populations of the diamondback moth, Plutella xylostella (L.). Molecular Ecology 15: 107-118.
Endersby NM, McKechnie SW, Vogel H, Gahan LJ, Baxter SW, Ridland PM and Weeks AR (2005). Microsatellites isolated from diamondback moth, Plutella xylostella (L.), for studies of dispersal in Australian populations. Molecular Ecology Notes 5: 51-53.
Endersby NM, Morgan WC, Stevenson BC and Waters CT (1992). Alternatives to regular insecticide applications for control of lepidopterous pests of Brassica oleracea var. capitata. Biological Agriculture and Horticulture 8: 189-203.
Endersby NM and Morgan WC (1991). Alternatives to synthetic chemical insecticides for use in crucifer crops. Biological Agriculture and Horticulture 8: 33-52.
Endersby NM, New TR and Thornton IWB (1990). Psocoptera from the Grampians and Mt. Arapiles, western Victoria – a biogeographic analysis. Journal of the Australian Entomological Society 29: 215-224.
Refereed Journals – co-author
Arnold, A., A. Kodym, N. M. Endersby-Harshman, J. Delpratt, and A. A. Hoffmann. 2017. Genetic structure of Gahnia radula (Cyperaceae), a key sedge for revegetation. Australian Journal of Botany 65: 128-139.
Ross, P. A., J. K. Axford, K. M. Richardson, N. M. Endersby-Harshman, and A. A. Hoffmann. 2017. Maintaining Aedes aegypti mosquitoes infected with Wolbachia. Journal of visualized experiments: JoVE.
Ross, P.A., I. Wiwatanaratanabutr, J.K. Axford, V.L. White, N.M. Endersby-Harshman, and A.A. Hoffmann, 2017, Wolbachia infections in Aedes aegypti differ markedly in their response to cyclical heat stress. PLOS Pathogens, 13 (1): p. e1006006.
Ross, P., N.M. Endersby, and A.A. Hoffmann, 2016, Costs of three Wolbachia infections on the survival of Aedes aegypti larvae under starvation conditions. PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004320 (2016), (1): p. e0004320.
H L Yeap, G Rašić, N M Endersby-Harshman, S F Lee, E Arguni, H Le Nguyen, A A Hoffmann: Mitochondrial DNA variants help monitor the dynamics of Wolbachia invasion into host populations. Heredity 11/2015; DOI:10.1038/hdy.2015.97
Gordana Rasic, Renata Schama, Rosanna Powell, Rafael Maciel-de Freitas, Nancy M. Endersby-Harshman, Igor Filipović, Gabriel Sylvestre, Renato C. Máspero, Ary A. Hoffmann: Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: Implications for vector control. Evolutionary Applications 08/2015; 8(9). DOI:10.1111/eva.12301
Ross, PA, Endersby, N. M., Yeap, H. L., & Hoffmann, A. A. (2014). Larval competition extends developmental time and decreases adult size of wMelPop Wolbachia-infected Aedes aegypti. American Journal of Tropical Medicine and Hygiene, 91(1), 198-205.
Rasic, G., Endersby NM, Williams C, & Hoffmann AA (2014). Using Wolbachia-based release for suppression of Aedes mosquitoes: insights from genetic data and population simulations. Ecological applications, 24 (5), 1226-1234.
Yeap HL, Axford JK, Popovici J, Endersby NM, Iturbe-Ormaetxe I, Ritchie SA, . . . Hoffmann AA (2014). Assessing quality of life-shortening Wolbachia-infected Aedes aegypti mosquitoes in the field based on capture rates and morphometric assessments. Parasites & Vectors 7, 13 pages. doi:10.1186/1756-305-7-58.
White VL, Endersby NM, Chan J, Hoffmann AA, & Weeks AR (2014). Developing Exon-Primed Intron-Crossing (EPIC) markers for population genetic studies in three Aedes disease vectors. Insect Science. doi:10.1111/1744-7917.12145.
Díaz F, Endersby NM & Hoffmann AA (2014). Genetic structure of the whitefly Bemisia tabaci populations in Colombia following a recent invasion. Insect Science. doi:10.1111/1744-7917.12129.
Olanratmanee P, Kittayapong P, Chansang C, Hoffmann AA, Weeks AR & Endersby NM 2013. Population genetic structure of Aedes (Stegomyia) aegypti (L.) at a micro-spatial scale in Thailand: implications for a dengue suppression strategy. PLoS Neglected Tropical Diseases 7, e1913.
Lee SF, White VL, Weeks AR, Hoffmann AA & Endersby NM (2012). High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans. Applied and Environmental Microbiology 78, 4740-4743.
Yeap HL, Mee P, Walker T, Weeks AR, O’Neill SL, Johnson P, Ritchie SA, Richardson KM, Doig C, Endersby NM, Hoffmann AA (2011). Dynamics of the ‘popcorn’ Wolbachia infection in outbred Aedes aegypti informs prospects for mosquito vector control. Genetics 187: 583-595.
Weeks AR, Endersby NM, Lange CL, Lowe A, Zalucki MP & Hoffmann AA (2010). Genetic variation among Helicoverpa armigera populations as assessed by microsatellites: a cautionary tale about accurate allele scoring. Bulletin of Entomological Research 100: 445–450.
Shelton AM, Gujar GT, Chen M, Rauf A, Srinivasan R, Kalia V, Mittal A, Kumari A, Ramesh K, Borkakatti R, Zhao JZ, Endersby N, Russell D, Wu YD & Uijtewaal B. (2009). Assessing the susceptibility of cruciferous Lepidoptera to Cry1Ba2 and Cry1Ca4 for future transgenic cruciferous vegetables. Journal of Economic Entomology 102 (6): 2217-2223.
Furlong MJ, Spafford H, Ridland PM, Endersby NM, Edwards OR, Baker GJ, Keller MA, Paull CA (2008). Ecology of diamondback moth in Australian canola: landscape perspectives and the implications for management. Australian Journal of Experimental Agriculture 48: 1494–1505.
Hamilton AJ, Endersby NM, Schellhorn NA, Ridland PM, Rogers PM, Jevremov D and Baker G (2006). Evaluation of fixed sample-size plans for Plutella xylostella (Lepidoptera: Plutellidae) on broccoli crops in Australia. Journal of Economic Entomology 99: 2171-2176.
Saw J, Endersby NM and McKechnie SW (2006). Low mtDNA diversity among widespread Australian diamondback moth Plutella xylostella (L.) suggests isolation and a founder effect. Insect Science 13: 237-241.
Hamilton AJ, Endersby NM, Ridland PM, Zhang J and Neal M (2005). Effects of cultivar on oviposition preference, larval feeding and development time of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), on some Brassica oleracea vegetables in Victoria. Australian Journal of Entomology 44: 284-287.
Hamilton AJ, Versace V, Hepworth G, Stagnitti F, Dawson J, Ridland PM, Endersby NM, Schellhorn NA, Mansfield C and Rogers PM (2005). Attending to risk in sequential sampling plans. In Environmental Health Risk III, C.A. Brebbia, V. Popov, & D. Fayzieva (eds.). The Sustainable World series, vol. 13. Wessex Institute of Technology. pp. 11-20.
Hamilton AJ, Schellhorn NA, Ridland PM, Endersby NM and Ward SA (2004). A dynamic binomial sequential sampling plan for diamondback moth, Plutella xylostella. Journal of Economic Entomology 96: 127-135.
Vickers R, Ridland PM and Endersby NM (2001). Australia leads the way in the fight against the diamondback moth. Pesticide Outlook 12: 185-187.
Conference Proceedings (Editor)
Endersby NM & Ridland PM eds (2004). The management of diamondback moth and other crucifer pests: Proceedings of the 4th International Workshop, Melbourne, Australia, 26-29 November 2001.
Endersby-Harshman NM (2016) From our special correspondent… The journalism of Arthur Ransome in Egypt. Amazon Publications, Kendal, Cumbria, United Kingdom, 310 pp.
Donald C, Endersby NM, Ridland PM, Porter I and Lawrence J (2000). Field guide to pests, diseases and disorders of vegetable brassicas. Department of Natural Resources and Environment, Victoria, Australia.
Andrew Hamilton, Vincent Versace, Graham Hepworth, Frank Stagnitti, Joanne Dawson, Peter M Ridland, Nancy M Endersby: Attending to risk in sequential sampling plans. Environmental Health Risk III, Edited by C.A. Brebbia, V. Popov, D. Fayzieva, 09/2005: pages 11-20; Wessex Institute of Technology., ISBN: ISBN: 1-84564-026-8
Full papers in Proceedings
Endersby NM (1991). Alternatives to synthetic insecticides: control of cabbage white butterfly and diamondback moth. Proceedings 1st National Conference Australian Society of Horticultural Science. Sustainable management of pests, diseases and weeds. Australian Horticulture Clean and Green in the 90’s. Macquarie University, Sydney, September 30 – October 3 1991, pp. 301-305.
Endersby NM (1991). Reduced use of synthetic insecticides: control of cabbage white butterfly and diamondback moth. Proceedings 1st National Conference Australian Society of Horticultural Science. Sustainable management of pests, diseases and weeds. Australian Horticulture Clean and Green in the 90’s. Macquarie University, Sydney, September 30 – October 3 1991, pp. 385-389.
Endersby NM (1992). Development of action thresholds for Pieris rapae (Lepidoptera: Pieridae) and Plutella xylostella (Lepidoptera: Yponomeutidae). Proceedings of the 5th Australian Applied Entomological Research Conference, Pest Control and Sustainable Agriculture, 27 April – 1 May 1992, Canberra, pp. 148-151.
Endersby NM and Ridland PM (1997). Insecticide resistance in diamondback moth, Plutella xylostella (L.) in southern Australia. In: The Management of Diamondback Moth and other Crucifer Pests: Proceedings of the Third International Workshop (eds A Sivapragasam, WH Loke, AK Hussan & GS Lim). Malaysian Agricultural Research and Development Institute, Kuala Lumpur, Malaysia, pp. 290-294.
Endersby NM, Ridland PM and Zhang J. (2004). Reduced susceptibility to permethrin in diamondback moth populations from vegetable and non-vegetable hosts in southern Australia. In: The management of diamondback moth and other crucifer pests: Proceedings of the Fourth International Workshop (eds NM Endersby & PM Ridland), 26 – 29 November 2001, Melbourne, Australia, pp. 319-325.
Endersby NM and Cameron PJ (2004). Parasitism of Nyctemera amica (White) (Lepidoptera: Arctiidae) and Plutella xylostella (L.) (Lepidoptera: Plutellidae) by Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae). In: The management of diamondback moth and other crucifer pests: Proceedings of the Fourth International Workshop (eds NM Endersby & PM Ridland), 26 – 29 November 2001, Melbourne, Australia, pp. 265-268.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2003). Development of genetic markers to study dispersal of diamondback moth, Plutella xylostella (L.) in Australia. In: Proceedings of the 13th Biennial Australian Research Assembly on Brassicas, Tamworth NSW, 8-12 Sep-2003, pp. 58-61.
Endersby NM, McKechnie SW and Ridland PM (2004). Population structure and movement of diamondback moth in Australia: beginnings of a molecular marker approach. In: Improving Biocontrol of Plutella xylostella. Proceedings of the International symposium (eds AA Kirk and D Bordat), CIRAD, Montpellier, France, 21-24 October 2002, pp. 167-171.
Endersby NM (2008). Population structure and gene flow in diamondback moth in Australia and around the world – current state of knowledge and directions for the future. In: Management of Diamondback Moth and Other Crucifer Pests: Proceedings of the Fifth International Workshop (eds AM Shelton, HL Collins,Y Zhang and Q Wu), Beijing China, October 24-27, 2006, China Agricultural Science and Technology Press, pp. 132-147.
Ridland PM and Endersby NM (2008). Diamondback Moth: Messages from a land down under. In: Management of Diamondback Moth and Other Crucifer Pests: Proceedings of the Fifth International Workshop (eds AM Shelton, HL Collins,Y Zhang and Q Wu), Beijing China, October 24-27, 2006, China Agricultural Science and Technology Press, pp. 1-29.
Ridland PM and Endersby NM (2008). Seasonal phenology of diamondback moth populations in southern Australia. In: Management of Diamondback Moth and Other Crucifer Pests: Proceedings of the Fifth International Workshop (eds AM Shelton, HL Collins,Y Zhang and Q Wu), Beijing China, October 24-27, 2006, China Agricultural Science and Technology Press, pp. 90-101.
Ridland PM and Endersby NM (2011). Some Australian populations of diamondback moth, Plutella xylostella (L.) show reduced susceptibility to fipronil. In: Proceedings of the Sixth International Workshop on Management of Diamondback Moth and Other Crucifer Pests (eds ), AVRDC World Vegetable Center, pp. 207-215.
Endersby NM & Ridland PM (1994). Insecticide resistance in Victorian populations of diamondback moth, Plutella xylostella (L.). Australian Entomological Society 25th Annual General Meeting and Scientific Conference. The University of Adelaide, 24-28 September 1994.
Endersby N, Ridland P, Heisswolf S and Houlding B (1996). Plutella – A resistant pest. Vegtec 2000. 1996 National Vegetable & Potato Industry Conference (AUSVEG), Brisbane Convention Centre, 7-9 July 1996.
Endersby NM, Ridland PM and Zhang J (2000) Resistance to permethrin in diamondback moth populations from vegetable and non-vegetable hosts in southern Australia.
Australian Entomological Society 31st AGM & Scientific Conference, Northern Territory University, Darwin, 25 – 30 June 2000.
Endersby NM, Ridland PM and Zhang J (2000) Resistance to permethrin in diamondback moth populations from vegetable and non-vegetable hosts in southern Australia. NRE Horticulture Conference, Institute for Horticultural Development, 6-7 September 2000.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2002). Population structure and movement of diamondback moth in Australia: beginnings of a molecular marker approach Australian Entomological Society 33rd AGM & Scientific Conference, Fremantle WA, 22-27-Sep-2002.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2003). Population structure and movement of diamondback moth in Australia: beginnings of a molecular marker approach. Department of Primary Industries and Department of Sustainability & Environment: Entomology Symposium. Rutherglen, May 7–8, 2003.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2003). Development of microsatellites to study population structure and movement of diamondback moth, Plutella xylostella (L.) in Australia, Australian Entomological Society 34th AGM & Scientific Conference, Hobart, Tasmania, 28 Sep-3 Oct –2003.
Endersby NM, McKechnie SW, Ridland PM and Weeks AR (2004). Population structure of Plutella xylostella (L.) investigated using microsatellite markers. XXII International Congress of Entomology, Brisbane, Queensland, Australia, 15-21 August 2004.
Endersby NM, White VL, Chan J, Hurst TP, Rašić G, Miller AD and Hoffmann AA (2012). Molecular markers provide evidence of cryptic species in Aedes notoscriptus. 11th Arbovirus Research In Australia. 10th Mosquito Control Association of Australia Symposium September 9 – 14, 2012. Outrigger Surfers Paradise, Surfers Paradise QLD, Australia.
Selection of Industry Publications
Endersby NM (1989). Cleaner Agriculture. Rural Quarterly, Spring 1989, Vol. 2, No. 3, p.18.
Endersby NM (1990). Companion Planting with insect repelling herbs. Crop Protection Bulletin, August 1990, No. 14, p. 22 & Geelong Advertiser, Tuesday 25 September, 1990.
Endersby NM (1990). Reduced synthetic insecticide inputs in vegetable growing. Crop Protection Bulletin, March 1990, No. 14, p. 14.
Endersby NM (1991). Reducing insecticide use in brassicas. Crop Protection Bulletin, September 1991, No. 26, p. 15.
Endersby NM (1991). Reduced use of synthetic insecticides: control of cabbage white butterfly and diamondback moth. Victorian Entomologist 21 (5): 127 – 129.
Endersby NM (1992). Entomopathogenic fungi. Natural biological control of brassica pests. Crop Protection Bulletin, September 1992, No. 37, pp. 18 – 19.
Endersby NM (1994). Insecticide resistance in cabbage moth. Victorian Vegetable Grower, No. 2, February, 1994, p. 1.
Endersby NM (1994). Further reports of insecticide resistance in cabbage moth. Victorian Vegetable Grower April 1994, pp. 4 – 5.
Endersby NM and Ridland PM (1995). Insecticide resistance in diamondback moths. A challenge for brassica growers. Good Fruit & Vegetables Vol.5 No. 9 (February 1995): 22-23.
Endersby NM and Ridland PM (1998). Diamondback moth (DBM) – What’s going on in the 1998/ 99 season? Access to Asian Vegetables Newsletter, Issue 13 (October 1998).
Endersby NM (1998). 1998/99 Diamondback moth insecticide resistance management strategy. Access to Asian Vegetables Newsletter, Issue 15 (December 1998).
Endersby NM (1999). Start monitoring for diamondback moth. Southern Farmer (p. 4 October 1998 – special Southern Farmer lift-out)
Endersby NM (2001). Dealing with diamondback moth. Southern Farmer, May 2001, p.22
Endersby NM (2001). Scouting for diamondback moth in brassicas. Vegetable Matters – NRE’s news for the Victorian vegetable industries Issue No. 1, June 2001.
Endersby NM (2003). Studying moth movement and insecticide resistance using molecular markers. Brassica IPM National Newsletter Issue 3, July 2003, pp. 2-3.
Endersby NM, McKechnie SW, Ridland PM and Weeks AR (2004). Australia-wide movement of diamondback moth studied using genetic markers. Brassica IPM National Newsletter Issue 5, September 2004, pp.2-3.
Endersby NM (2006) Genetic structure and insecticide resistance in Australian DBM. Brassica IPM National Newsletter Issue 9, August 2006, pp. 3-4.
2016 Invitrogen Science Hero (Behind the scenes category)
2005 Department of Primary Industries Daniel McAlpine Outstanding Achievement Award (Member of Sampling Team)
Working with the PEAR group for many years now, I have both witnessed and participated in its incredible evolution. My work debut involved the miniscule but “mighty in damage” grapevine pest phylloxera and its plant hosts and I quickly learnt to adapt to large scale and often complicated (damaged insects and fibrous roots) genotyping. I also managed the Molecular lab for a short time and was instrumental in the development and implementation of training (equipment and procedural) and EHS practises.
It is fitting that after I became a mother. I returned to the group to work on “the Mother of all dengue vectors”, Aedes aegypti. Here I worked closely with an incredible and inspiring mentor, Nancy Endersby-Harshman, to develop and test genetic markers in Aedes aegypti but also Aedes notoscriptus and Aedes albopictus. Research applications for these markers included population genetics, phylogeography, phenotypic trait association and conversion to new technology platforms (for example, from the P33 radioisotope to the fluorescence based system for microsatellite markers).
Then an endosymbiotic bacterium called Wolbachia exploded into my working life and it was both incredibly promising in terms of its ability to block the dengue virus (and later zika virus) and complex because it was a “novel” vector control strategy with the option of multiple Wolbachia strains whose dengue blocking and host effects (negative and positive) varied. PEARG first focused on the Wolbachia strain wMel and a dedicated period of colony experiments, field releases, molecular testing and post-release monitoring followed providing us with a good understanding of its role in disease suppression. The PEARG captaincy by Professor Ary Hoffmann however soon added other mosquito hosts and Wolbachia strains into the mix and fine-tuned our research goals and approaches to better understand the genetic mechanisms both driving and confounding the spread of Wolbachia in natural populations.
My contributions to this “Wolbachia era” include screening of thousands of Aedes samples for Wolbachia infection status with established “high throughput” light cycler assays but also developing and optimising new light cycler assays including those that can detect very low level and multiple Wolbachia infections in mosquitoes and fruit flies. But by far the most rewarding experiences have been sharing these methodologies with visiting collaborators from China, Indonesia and Sri Lanka and my visit to the wonderful Institute of Medical Research (IMR) in Kuala Lumpur, Malaysia, where I helped adapt our procedures to their working environment. The importance of this IMR collaboration was further highlighted by colleagues who had been infected by dengue three and four times. A job that has the potential to help the lives of many people is something rare and special as is working for Ary, who believes in sharing and advancing the technology and resources that he and his team have developed to fight dengue where it is truly needed.
Recently I hopped over to grasshoppers: So far I have tested mitochondrial and nuclear markers on the parthenogenetic species Warramaba virgo (comprised of two distinct phylads, Standard and Boulder-Xanthus) and maintained a colony of and performed physiological trait measurements on Vandiemenella (another genus of Australian grasshoppers that is made up of different chromosome races). I am now learning about the fascinating Keyacris scurra grasshopper and its conservation plight that restricts it to a handful of regional graveyards. And I am also exploring potential next generation sequencing technologies that can be used to characterise the genetic diversity and evolutionary histories of matchstick grasshoppers (family Morabidae, comprised of over 300 species that do not occur outside Australia) and help identify threats to their future (for example, climate change) and practises that can reduce those threats (for example, translocations of individuals to threatened populations).
Keyacris scurra walks into a Melbourne Uni lab and the scientist says “Why the long face?” and she answers “Because I am at grave risk of extinction”.
*Photo credit: Michael Kearney.
*Molecular training of overseas visitors from Iran and China (mosquito project).
*Development of nuclear SNP markers to detect clonal variation in the parthenogenetic grasshopper Warramaba virgo.
*Ongoing colony maintenance and physiological trait measurements (such as feeding rate) in Vandiemenella grasshoppers.
*Exploring suitable next generation sequencing technologies for understanding the evolutionary histories of and current threats to Morabidae grasshoppers.
Umina, P. A., Corrie, A. M., Herbert, K. S., White, V. L., Powell, K. S. and Hoffmann, A. A. (2007) The use of DNA markers for pest management -clonal lineages and population biology of grape phylloxera. Acta. Hortic.,733, 183-189.
Endersby, N. M., Hoffmann, A. A., White, V. L., Lowenstein, S., Ritchie, S., Johnson, P. H., Rapley, L. P., Ryan, P. A., Nam, V. S., Yen, N. T., Kittiyapong, P. and Weeks, A. R. (2009) Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and Exon-Primed Intron-Crossing markers suggests feasibility of local control options. Journal of Medical Entomology, 46, 1074-1083.
Endersby, N. M., Hoffmann, A. A., White, V. L., Ritchie, S. A., Johnson, P. H. and Weeks, A. R. (2011) Changes in the genetic structure of Aedes aegypti (Diptera: Culicidae) populations in Queensland, Australia, across two seasons: Implications for potential mosquito releases. Journal of Medical Entomology, 48, 999-1007.
Lee, S. F., White, V. L., Weeks, A. R., Hoffmann, A. A. and Endersby, N. M. (2012) High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans. Appl. Environ. Microbiol. 78(13), 4740-4743.
Endersby, N. M., White, V. L., Chan, J., Hurst, T., Rašić, G., Miller, A. and Hoffmann, A. A. (2013) Evidence of cryptic genetic lineages within Aedes notoscriptus (Skuse). Infection, Genetics and Evolution, 18, 191-201.
Rakimov, A., Ben-Dov, Y., White, V. and Hoffmann, A. A. (2013) Soft scale insects (Hemiptera: Coccoidea: Coccidae) on grapevines in Australia. Australian Journal of Entomology, 52, 371-378.
White, V. L., Endersby, N. M., Chan, J., Hoffmann, A. A and Weeks, A. R. (2014) Developing Exon-primed intron-crossing (EPIC) markers for population genetic studies in three Aedes disease vectors. Insect Science, 22(3), 409-423.
Wuliandari, J. R., Lee, S. F., White, V. L., Tantowijoyo, W., Hoffmann, A. A. and Endersby-Harshman, N. M. (2015) Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia. Insects, 6, 658-685.
Rasic, G., Endersby-Harshman, N., Tantowijoyo, W., Goundar, A., White, V., Qiong, Y., Filipovic, I., Johnson, P., Hoffmann, A. and Arguni, E. (2015) Aedes aegypti has spatially structured and seasonally stable populations in Yogyakarta, Indonesia. Parasites & Vectors, 8, 610.
Hancock, P. A., White, V. L., Callahan, A. G., Godfray, C. H. J., Hoffmann, A. A. and Ritchie, S. A. (2016) Density-dependent population dynamics in Aedes aegypti slow the spread of wMel Wolbachia. Journal of Applied Ecology, 53(3): 785-793.
Hancock, P. A., White, V. L., Ritchie, S. A., Hoffmann, A. A. and Godfrey, H. C. J. (2016) Predicting Wolbachia invasion dynamics in Aedes aegypti populations using models of density-dependent demographic traits. BMC Biology, 14, 96.
Ross, P. A., Wiwatanaratanabutr, I., Axford, J. K., White, V. L., Endersby-Harshman, N. M. and Hoffmann, A. A. (2016) Wolbachia Infections in Aedes aegypti differ markedly in their response to cyclical heat stress. PLOS Pathogens, 13(1): e1006006.
Melissa Carew, Research Fellow
Melissa’s main interests are understanding species diversity using DNA barcodes and developing DNA-based tools for environmental monitoring, especially in freshwater ecosystems. In the past, her research has largely focused on using DNA approaches to understand life histories, population structure and for species identification of various invertebrate (and some vertebrate) groups. During her PhD, she developed DNA approaches for species identification of Chironomidae (Diptera), well known as a bioindicator group in freshwater environments. She also investigated the bioindicator potential in particular chironomid species by examining their field-based responses to pollution. More recently, her work has broadened to include identifying many species of freshwater macroinvertebrates using DNA barcoding.
- Improved biomonitoring of urban freshwater ecosystems using DNA barcodes -ARC Linkage project with Melbourne Water Corporation (LP150100876)
Jeppe, K. J., Carew, M. E., Pettigrove, V., and Hoffmann, A. A. (2017). Toxicant mixtures in sediment alter gene expression in the cysteine metabolism of Chironomus tepperi. Environmental Toxicology and Chemistry 36, 691-698.
Carew, M., Nichols, S., Batovska, J., St Clair, R., Murphy, N., Blacket, M., and Shackleton, M. (2017). A DNA barcode database of Australia’s freshwater macroinvertebrate fauna. Marine and Freshwater Research. Early online.
Jeppe, K. J., Yang, J., Long, S. M., Carew, M. E., Zhang, X., Pettigrove, V., Hoffmann, A. A., and Piggott, J. (2016). Detecting copper toxicity in sediments: from the subindividual level to the population level. Journal of Applied Ecology. Early online.
Carew, M. E., Metzeling, L., St Clair, R., and Hoffmann, A. A. (2016). Detecting invertebrate species in archived collections using next-generation sequencing. Molecular Ecology Resources. Early online.
Carew, M. E., Hoffmann, A. A. (2015). Delineating closely related species with DNA barcodes for routine biological monitoring. Freshwater Biology 60, 1545-1560.
Jeppe, K. J., Carew, M. E., Long, S. M., Lee, S. F., Pettigrove, V., and Hoffmann, A. A. (2014). Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium. Comparative Biochemistry Physiology C Toxicology and Pharmacology 162, 1-6.
Proulx, I., Martin, J., Carew, M., and Hare, L. (2013). Using various lines of evidence to identify Chironomus species.(Diptera: Chironomidae) in eastern Canadian lakes. Zootaxa 3741, 401-458.
Carew, M. E., Pettigrove, V. J., Metzeling, L., and Hoffmann, A. A. (2013a). Environmental monitoring using next generation sequencing: Rapid identification of macroinvertebrate bioindicator species. Frontiers in Zoology 10, 45.
Carew, M., Gagliardi, B., and Hoffmann, A. (2013b). Mitochondrial DNA suggests a single maternal origin for the widespread triploid parthenogenetic pest species, Paratanytarsus grimmii, but microsatellite variation shows local endemism. Insect Science 20, 345-357.
Qian, Z-Q., Sara Ceccarelli, F., Carew, M. E., Schl√ºns, H., Schlick-Steiner, B. C., and Steiner, F. M. (2011). Characterization of polymorphic microsatellites in the giant bulldog ant, Myrmecia brevinoda and the jumper ant, M. pilosula. Journal of Insect Science 11, 1-8.
Carew, M. E., Miller, A. D., and Hoffmann, A. A. (2011a). Phylogenetic signals and ecotoxicological responses: Potential implications for aquatic biomonitoring. Ecotoxicology 20, 595-606.
Carew, M. E., Marshall, S. E., and Hoffmann, A. A. (2011b). A combination of molecular and morphological approaches resolves species in the taxonomically difficult genus Procladius Skuse (Diptera: Chironomidae) despite high intra-specific morphological variation. Bulletin of Entomological Research 101, 1-15.
O’Brien ML, Pettigrove, V., Carew, M. E., Hoffmann, A. A. (2010). Combining rapid bio assessment and field-based microcosms for identifying impacts in an urban river. Environmental Toxicology and Chemistry 29, 1773-1780.
Marshall S, Pettigrove, V., Carew, M., Hoffmann A (2010). Isolating the impact of sediment toxicity in urban streams. Environmental Pollution 158, 1716-1725.
Coleman R, Pettigrove, V., Raadik T, Hoffmann A, Miller A, Carew M (2010). Microsatellite markers and mtDNA data indicate two distinct groups in dwarf galaxias, Galaxiella pusilla; (Mack) (Pisces: Galaxiidae), a threatened freshwater fish from south-eastern Australia. Conservation Genetics 11, 1911-1928.
Townsend, K. R., Pettigrove, V. J., Carew, M. E., and Hoffmann, A. A. (2009). The effects of sediment quality on benthic macroinvertebrates in the River Murray, Australia. Marine and Freshwater Research 60, 70-82.
Schiffer, M., Umina, P., Carew, M., Hoffmann, A., Rodoni, B., and Miller, A. (2009). The distribution of wheat curl mite (Aceria tosichella) lineages in Australia and their potential to transmit wheat streak mosaic virus. Annals of Applied Biology 155, 371-379.
Carew, M., Schiffer, M., Umina, P., Weeks, A., and Hoffmann A (2009). Molecular markers indicate that the wheat curl mite, Aceria tosichella Keifer, may represent a species complex in Australia. Bulletin of Entomological Research 99, 479-486.
Anson, J., Pettigrove, V., Carew, M. E., and Hoffmann, A. A. (2008). High molecular weight petroleum hydrocarbons derived from different sources affect freshwater benthic macroinvertebrate assemblages. Environmental Toxicology and Chemistry 27, 1077-1083.
van Zweden, J., Carew, M., Henshaw, M., Robson, S., Crozier, R. (2007). Social and genetic structure of a supercolonial weaver ant, Polyrhachis robsoni , with dimorphic queens. Insectes Sociaux 54, 34-41.
Cox, R., Hoffmann, A. A., Pettigrove, V., and Carew ME (2007). A microcosm approach for assessing the response of indigenous chironomids to salinity. Australasian Journal of Ecotoxicology 13, 113-118.
Carew, M. E., Pettigrove, V., Cox, R. L., and Hoffmann, A. A. (2007a). The response of Chironomidae to sediment pollution and other environmental characteristics in urban wetlands. Freshwater Biology 52, 2444-2462.
Carew, M. E., Pettigrove, V., Cox RL, and Hoffmann, A. A. (2007b). DNA identification of urban Tanytarsini chironomids (Diptera: Chironomidae). Journal of the North American Benthological Society 24, 586-599.
Carew, M. E., Pettigrove, V., and Hoffmann, A. A. (2005). The utility of DNA markers in classical taxonomy: Using cytochrome oxidase I markers to differentiate Australian Cladopelma (Diptera : Chironomidae) midges. Annals of the Entomological Society of America 98, 587-594.
Carew, M. E., Goodisman, M. A. D., and Hoffmann, A. A. (2004). Species status and population genetic structure of grapevine eriophyoid mites. Entomologia Experimentalis Et Applicata 111, 87-96.
Schiffer, M., Carew, M. E., and Hoffmann, A. A. (2004). Molecular, morphological and behavioural data reveal the presence of a cryptic species in the widely studied Drosophila serrata species complex. Journal of Evolutionary Biology 17, 430-442.
Thomson, L. J., Rundle, B. J., Carew, M. E., and Hoffmann, A. A. (2003). Identification and characterization of Trichogramma species from south-eastern Australia using the internal transcribed spacer 2 (ITS-2). region of the ribosomal gene complex. Entomologia Experimentalis et Applicata 106, 235-240.
Carew, M. E., Pettigrove, V., and Hoffmann, A. A. (2003). Identifying chironomids (Diptera: Chironomidae). for biological monitoring with PCR-RFLP. Bulletin of Entomological Research 93, 483-490.
Magiafoglou, A., Carew, M. E., and Hoffmann, A. A. (2002). Shifting clinal patterns and microsatellite variation in Drosophila serrata populations: a comparison of populations near the southern border of the species range. Journal of Evolutionary Biology 15, 763-774.
Goodisman, M. A. D., Matthews, R. W., Spradbery, J. P., Carew, M. E., and Crozier, R. H. (2001). Reproduction and recruitment in perennial colonies of the introduced wasp Vespula germanica. Journal of Heredity 92, 346-349.
Crozier, R. H., Kaufmann, B., Carew, M. E., Crozier, Y. C. (1999). Mutability of microsatellites developed for the ant Camponotus consobrinus. Molecular Ecology 8, 271-276.
Crozier, R. H., Oldroyd, B. P., Tay, W. T., Kaufmann, B. E., Johnson, R. N., Carew, M. E., and Jennings, K. M. (1997). Molecular advances in understanding social insect population structure. Electrophoresis 18, 1672-1675.
Carew M. E., Tay W. T., and Crozier R. H. (1997). Polygyny via unrelated queens indicated by mitochondrial DNA variation in the Australian meat ant, Iridomyrmex purpureus. Insectes Sociaux 44, 7-14.
2006 PhD – The University of Melbourne
1996 B.Sc (Hon) La Trobe University
Garry McDonald, Research Fellow
Garry has been a research and extension entomologist with the Victorian DPI for 22 years, working initially on mosquito and ant ecology /management, and subsequently on integrated pest management (IPM) and biological control strategies for a range of pests of horticulture, field crops and pastures. Some focal pests of particular interest included Helicoverpa spp. (native budworm and corn earworm), Leucania and Persectania spp. (armyworm), Agrotis spp. (cutworm), pasture cockchafers, Nysius vinitor (Rutherglen bug) and earth mites.
Since 1996, Garry assumed broader administrative roles as Director, Rutherglen Research Institute, subsequently Research Director of DPIs Landscape Systems Sciences Platform, and more recently Director of Swinburne University’s National Centre for Sustainability. In this capacity, he was a Board Director for the Energy Efficiency Council for two years.
Garry joined PEARG in mid-2012 in a part time capacity to undertake research aimed at developing pest forecasting models for the key pests of the crop establishment phase of Australian grain crops – Halotydeus destructor (redlegged earth mite) and Sminthurus viridis (lucerne flea). The modelling focus on developing predictions of both the timing of outbreaks, and their relative severity. He is also the Executive Officer for the Grains Pest Advisory Committee, a national science advisory group for the Grains Research and Development Corporation.
- National Coordination of Invertebrate Pest Research and Insecticide Resistance Management, and the Grains Pest Advisory Committee
Macfadyen, S., McDonald, G., Hill, M. P., (2016). From species distributions to climate change adaptation: Knowledge gaps in managing invertebrate pests in broad-acre grain crops. Agriculture, Ecosystems & Environment. https://doi.org/10.1016/j.agee.2016.08.029
McDonald, G., Umina, P. A., MacFadyen, S., Mangano, P., and Hoffmann, A. A. (2015). Predicting the timing of first generation egg hatch for the pest redlegged earth mite Halotydeus destructor (Acari: Penthaleidae). Experimental and Applied Acarology 65: 259-276 DOI 10.11007/s10493-014-9876-x
Tom joined PEARG in 2014 to undertake a PhD, and since finishing in 2018 is currently employed as a research fellow. His research considers the invasive arbovirus vectors Aedes aegypti (yellow fever mosquito) and Aedes albopictus (Asian tiger mosquito), with an aim to understand how they disperse through anthropogenic environments. This research informs biocontrol efforts at three key stages: identifying invasion routes and source populations, estimating the potential for spatial spread of disease during arbovirus outbreaks, and designing strategies for the elimination or control of invasive populations.
PEARG has been central to the development of vector control strategies involving the release of mosquitoes infected with the bacterium Wolbachia, which leads to either local population eradication or the ongoing suppression of its vectorial capacity. Tom has been involved in these developments directly, investigating spatial dynamics of the three Wolbachia invasions established among Cairns Aedes aegypti in 2013.
- Genetic structure among Asian Aedes albopictus
- Genetic structure among Aedes mosquitoes in and around the Torres Strait
- Bioinformatics as a tool to trace invasion pathways of invasive species
Local introduction and heterogeneous spatial spread of dengue-suppressing Wolbachia through an urban population of Aedes aegypti
TL Schmidt, NH Barton, G Rašić, AP Turley, BL Montgomery, et al.
PLoS Biology 15 (5), e2001894
Genome-wide SNPs reveal the drivers of gene flow in an urban population of the Asian Tiger Mosquito, Aedes albopictus
TL Schmidt, G Rašić, D Zhang, X Zheng, Z Xi, AA Hoffmann
PLoS neglected tropical diseases 11 (10), e0006009
Fine-scale landscape genomics helps explain the slow spread of Wolbachia through the Aedes aegypti population in Cairns, Australia
TL Schmidt, I Filipović, AA Hoffmann, G Rašić
ASTMH Annual Meeting 2016 – Poster Presenter
Australian Entomology Society Conference 2017 – 10 minute talk
Matthew Binns, Research Assistant
Matthew is currently working at PEARG as an entomologist with a focus on agricultural pest management. His background has been mostly based at the University of New England where, from 2008, he studied insect morphology and species diversity over climatic gradients. From 2012, Matthew shifted focus into agricultural issues where he studied attract-and-kill management strategies for Helicoverpa moths in cotton and diamondback moth in canola.
- Insecticide resistance management in red legged earth mites
- Identifying the common earwig species present in grain production systems and classifying them as pests or beneficials
- Quantifying the impact of beneficials on pest populations in grain production systems
Gregg, P., Del Socorro, A.P., and Binns, M. R. (2016). Non-target impacts of an attract-and-kill formulation based on plant volatiles: responses of some generalist predators. Journal of chemical ecology 42.7, 676-688.
Gregg, P., Del Socorro, A.P., Hawes, A.J., and Binns, M. R. (2016). Developing bisexual attract-and-kill for polyphagous insects: ecological rationale versus pragmatics. Journal of chemical ecology 42.7, 666-675.
Gibb, H., Muscat, D., Binns, M. R., Silvey, C. J., Peters, R. A., Warton D. I., and Andrew, N. R. (2015). Responses of foliage-living spider assemblage composition and traits to a climatic gradient in Themeda grasslands, Austral Ecology 40(3), 225-237.
Brown, A. M. Warton, D. I., Andrew, N. R., Binns, M., Cassis, G., and Gibb, H (2014). The fourth-corner solution – using predictive models to understand how species traits interact with the environment. Methods in Ecology and Evolution 5(4), 344-352.
Yates, M. L., Andrew, N. R., Binns, M., and Gibb, H (2014). Morphological traits: predictable responses to macrohabitats across a 300 km scale, PeerJ 2: e271.
Andrew, N. R., Hill, S. J., Binns, M. R., Bahar, M. H., Ridley, E. V., Jung, M. P., Fyfe, C., Yates, M., and Mohammad Khusro (2013). Assessing insect responses to climate change: What are we testing for? Where should we be heading, PeerJ 1: e11.
2015 Predicting the effect of climate change on community structure and function: an assessment using grassland thrips. Ecostats Conference, Sydney, Australia.
2014 Helicoverpa punctigera: Local and Inland overwintering biology. REFCOM conference, Goondiwindi, Australia.
2012 International Congress of Entomology, Daegu, South Korea.
2011 Ecological Society of Australia Conference, Hobart, Australia.
2010 Ecological Society of Australia Conference, Canberra, Australia.
2008 Importance of climate and plant trait variables in structuring beetle communities on Acacia. The Ecological Society of Australia Conference, Sydney, Australia.
I have a BSc joint honours degree in Biochemistry and Pharmacology from King’s College, University of London and an MSc in Natural Resource Management from the University of Leicester, UK. I obtained my PhD in aquatic ecotoxicology from RMIT-University in 2001 where I investigated the effects of crude oil on octopus and mussels. I spent eight years as a terrestrial ecotoxicologist at the Centre for Ecology and Hydrology at Monks Wood in the UK, investigating the effects of pesticides and industrial contaminants on vertebrates and invertebrates using traditional and novel biochemical techniques. I am currently a Research Fellow at CAPIM and PEARG in the School of BioSciences at the University of Melbourne. I have supervised two PhD students (conferred in 2017), three Masters students and a post-graduate diploma student at the University of Melbourne. I am currently supervising two PhD students within the School of BioSciences.
My main research focus is to develop novel methods (predominantly metabolomics, proteins and enzyme activity) to assess biological effects of environmental stressors (anthropogenic and natural) in aquatic organisms. These methods can be used in biomonitoring programs alongside analytical chemistry techniques and community surveys to provide early warning indications of poor water quality to assist in the management of aquatic ecosystems. I am particularly interested in the interaction between stressors and organisms at the individual level to gain a greater understanding of the mechanisms of action of these stressors. This will allow us to better predict adverse effects of mixtures of stressors in the future. I am currently working on a project to develop novel methods of assessing pollution stress in estuarine environments using metabolomics and DNA metabarcoding in collaboration with Melbourne Water and CSIRO.
Bryant S. Gagliardi, Sara M. Long, Vincent J. Pettigrove, Ary A. Hoffmann (2015). The parthenogenetic cosmopolitan chironomid, Paratanytarsus grimmii, as a new standard test species for ecotoxicology: culturing methodology and sensitivity to aqueous pollutants. Bulletin of Environmental Contamination and Toxicology 95 (3) 350-356
Sara M. Long, Dedreia L. Tull, Katherine J. Jeppe, David P. De Souza, Saravanan Dayalan, Vincent J. Pettigrove, Malcolm J. McConville, Ary A. Hoffmann (2015). A multi-platform metabolomics approach demonstrates changes in energy metabolism and the transsulfuration pathway in Chironomus tepperi following exposure to zinc. Aquatic Toxicology 162, 54-65
Katherine J. Jeppe, Melissa E. Carew, Sara M. Long, Siu F. Lee, Vincent Pettigrove, Ary A. Hoffmann. (2014). Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium. Comparative Biochemistry and Physiology Part C. 162, 1-6.
Claudette Kellar, Kathryn L. Hassell, Sara M. Long, Jackie Myers, Lisa Golding., Gavin Rose, Anupama Kumar, Ary Hoffmann, Vincent Pettigrove (2014). Multiple ecological evidence links adverse biological effects to pesticide and metal contamination in an urban Australian watershed. Journal of Applied Ecology. 51 (2), 426-439.
Oliver A.H. Jones,, Steven Murfitt, Claus Svendsen, Anthony Turk, Hazel Turk, David J. Spurgeon, Lee A. Walker, Richard F. Shore, Sara M. Long, Julian L. Griffin. (2013). Comparisons of metabolic and physiological changes in rats following short term oral dosing with pesticides commonly found in food. Food and Chemical Toxicology. 59: 438-445
Fredrik Reichenberg, Ulrich Gosewinkel Karlson, Orjan Gustafsson, Sara M. Long, Parmely H. Pritchard, Philipp Mayer. (2010). Low accessibility and chemical activity of PAHs restrict bioremediation and risk of exposure in a manufactured gas plant soil. Environmental Pollution 158: 1214-1220 (20)
Sara M. Long, Fredrik Reichenberg, Lindsay J. Lister, Peter K. Hankard, Jo Townsend, Philipp Mayer, Julian Wright, Martin Holmstrup, Claus Svendsen, David J. Spurgeon. (2009). Combined chemical (fluoranthene) and drought effects on Lumbricus rubellus demonstrates the applicability of the independent action model for multiple stressor assessment. Environmental Toxicology and Chemistry 28 (3), 629-636
Spurgeon DJ, Rowland P, Ainsworth G, Rothery P, Long S, Black HIJ. (2008). Geographical and pedological drivers of distribution and risks to soil fauna of seven metals (Cd, Cu, Cr, Ni, Pb, V and Zn) in British soils.Environmental Pollution 153: 2, 273-283
Walker, L.A, Turk, A., Long, S.M., Wienburg, C.L., Best, J., & Shore, R.F. (2008). Second generation anticoagulant rodenticides in tawny owls (Strix aluco) from Great Britain. The Science of the Total Environment 392 93-98
- Heywood, J. Wright, C.L. Wienburg, H.I.J. Black, S.M. Long, D. Osborn and D.J. Spurgeon. (2006) Factors Influencing the National Distribution of Polycyclic Aromatic Hydrocarbons and Polychlorinated Biphenyls in British Soils. Environmental Science and Technology 40 7629-7635
SM Long, A. Dawson and RF Shore (2006). A comparison of the effects of single and repeated exposure to an organophosphate insecticide on acetylcholinesterase activity in mammals. Environmental Toxicology and Chemistry 25 (7) 1857-1863
Brown, P.J., Long, S.M., Spurgeon, D.J., Svendsen, C. and Hankard, P. (2004) Toxicological and biochemical responses of the earthworm Lumbricus rubellus to pyrene, a non-carcinogenic polycyclic aromatic hydrocarbon. Chemosphere 57 (11) 1675-1681
SM Long, KJ Ryder and DA Holdway (2003). The Use of Respiratory Enzymes as Biomarkers of Petroleum Hydrocarbon Exposure in Mytilus edulis planulatus. Ecotoxicology and Environmental Safety 55 (3), 261-270
SM Long and DA Holdway (2002). Acute Toxicity of crude and dispersed crude oil to Octopus pallidus (Hoyle, 1885) hatchlings. Water Research 36 (11), 2769-2776.
(presented at national and international conferences, University of Melbourne departmental seminars, stakeholder meetings and research summits; a selection of the most recent presentations is listed below)
Sara M. Long, Dedreia L. Tull, David P. de Souza, Konstantinos A. Kouremenos, Saravanan Dayalan, Jarrad K. Baker, Kathryn L. Hassell, Malcolm J. McConville, Vincent J. Pettigrove, Marthe Monique Gagnon. Environmental metabolomics provides insights into physiological responses of Southern Sand Flathead in Port Phillip Bay. Platform presentation in Special Session on Environmental Stress and its effects on fish at Australian Society of Fish Biology, 21st-24th July 2017, Albany, Australia.
Allyson O’Brien, Sara Long, Anthony Chariton, Mick Keough, Rhys Coleman, Dedreia Tull, Malcolm McConville. Using metabarcoding and metabolomics to measure marine community structure and function. Platform presentation at Australian Marine Science Conference 2017, 2nd-6th July, Darwin, Australia.
Sara M. Long, Georgia M. Sinclair, Allyson L. O’Brien, David P. De Souza, Konstantinos A Kouremenos, Saravanan Dayalan, Ary A. Hoffmann, Michael J. Keough, Malcolm J. McConville, Dedreia L. Tull. Can metabolomics approaches be used to determine the impact of pollution in estuarine environments? Platform presentation at Metabolomics Society conference 26th-29th June 2017, Brisbane, Australia.
Georgia M. Sinclair, Allyson L. O’Brien, Mick Keough, David P. De Souza, Saravanan Dayalan, Dedreia L. Tull and Sara M. Long. Using Metabolomics to Assess the Early Effects of Zinc and Boscalid On Estuarine Polychaete. Platform presentation at Metabolomics Society conference 26th-29th June 2017, Brisbane, Australia.
Dedreia L. Tull, Sara M. Long, David P. de Souza, Konstantinos A. Kouremenos, Jarrad K. Baker, Kathryn L. Hassell, Malcolm J. McConville, Vincent J. Pettigrove, Marthe Monique Gagnon. Environmental metabolomics provides insights into physiological responses of Southern Sand Flathead in Port Phillip Bay. Poster presentation at Metabolomics Society conference 26th-29th June 2017, Brisbane, Australia.
Bryant Gagliardi, Sara Long, Vincent Pettigrove, Ary Hoffmann. Chironomid deformities: are they actually induced by aquatic pollution? Poster presentation at SETAC Europe conference, 7th – 11th May 2017, Brussels, Belgium.
Sara Long and Allyson O’Brien. Developing new tools for monitoring estuarine pollution. Invited presentation at Melbourne Water Waterways Research Stakeholder Forum, December 2nd 2016.
Sara M Long, Georgia M. Sinclair, Allyson L. O’Brien, Dedreia L. Tull, David P. De Souza, Konstantinos A Kouremenos, Saravanan Dayalan, Ary A. Hoffmann, Malcolm J. McConville. Multi-species metabolomics to enable the design of cost-effective biomonitoring programs for determining the impacts of pollution in estuarine environments. Platform presentation at SETAC Europe/UK Environmental Omics Synthesis Centre symposium on environmental and (eco)toxicological Omics and Epigenetics, 12th-15th Sept 2016, Ghent, Belgium.
University of Melbourne/University of Birmingham Travel award 2016
Inaugural SETAC-Au Travel Fellowship 2016
Centre for Ecology and Hydrology New Blood Fellow 2001-2004
I majored in Zoology and Genetics with Honours at Latrobe University, Bundoora and the University of Melbourne. During my honours year in 2009, I developed skills in Drosophila melanogaster DNA sequence analysis and cloning techniques. My professional career began in 2010 as a Research Officer within the PEARG. My skills have expanded since 2010 to include insect husbandry (Aedes aegypti & Aedes notoscriptus), method design and fitness experimentation upon Aedes aegypti, the primary vector of the Dengue virus. The research aims to inform population dynamics models which predict the spread of released mosquitoes artificially infected with an intracellular bacterium, called Wolbachia pipientis, the goal being arbovirus transmission cycle disruption between humans. Other research has involved species distribution modelling upon Aedes albopictus, field work in Cairns and abroad, respirometry, elucidating the transmission cycle of Mycobacterium ulcerans and thermocycler heat stress assays.
NHMRC Dengue Program
Molecular Basis of Adaptive Shift in Body Size in Drosophila melanogaster: Functional and Sequence Analyses of the Dca Gene
Siu F Lee, Ying Chen, Aiden K Varan, Choon W Wee, Ary A Hoffmann
The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations
T Walker, P.H. Johnson, L.A. Moreira, I Iturbe-Ormaetxe, AA Hoffmann
Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission
A.A. Hoffmann, B.L. Montgomery, J Popovici, I Iturbe-Ormaetxe, S L O’Neill
Predicting the spread of Aedes albopictus in Australia under current and future climates: Multiple approaches and datasets to incorporate potential evolutionary divergence
Matthew P. Hill, Jason K. Axford, Ary A. Hoffmann
Assessing quality of life-shortening Wolbachia-infected Aedes aegypti mosquitoes in the field based on capture rates and morphometric assessments
Heng Lin Yeap, Jason K Axford, Jean Popovici, Nancy M Endersby, Ary A Hoffmann
Stability of the wMel Wolbachia Infection following Invasion into Aedes aegypti Populations
Ary A Hoffmann, Inaki Iturbe-Ormaetxe, Ashley G Callahan, Ben L Phillips, Scott L O’Neill
Fitness of wAlbB Wolbachia Infection in Aedes aegypti: Parameter Estimates in an Outcrossed Background and Potential for Population Invasion
Jason K Axford, Perran A Ross, Heng Lin Yeap, Ashley G Callahan, Ary A Hoffmann
Novel applications of thermocyclers for phenotyping invertebrate thermal responses
Jacinta D. Kong, Jason K. Axford, Ary A. Hoffmann, Michael R. Kearney
Wolbachia infections in Aedes aegypti differ markedly in their response to cyclical heat stress
Perran Ross, Itsanun Wiwatanaratanabutr, Jason Axford, Vanessa White, Ary Hoffmann
Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer
John R. Wallace, Kirstie M. Mangas, Jessica L. Porter, Renee Marcsisin, Timothy P. Stinear
Maintaining Aedes aegypti mosquitoes infected with Wolbachia
Perran A. Ross, Jason K. Axford, Kelly M. Richardson, Nancy M. Endersby-Harshman, Ary A. Hoffmann
Ary keeps me busy managing the lab’s business affairs, occasionally I find time to do a spot of research. I came to science a bit late in the game after several incarnations (audio engineer, archivist, landscape gardener, removalist, ward clerk, night clerk, risk assessor etc.). I completed a bachelor of biological sciences at LaTrobe in 2013, jumped ship to complete an honours year at Melbourne and managed to pull off a first class result under Ary’s supervision. I spent a couple years as a research assistant doing a mix of agricultural consulting work on the road and in the lab. I have a diverse repertoire of skills from bioinformatics to field ecology, lab assays and many points in between. Jack of all trades, master of some. Most of my functions these days are focused on running the labs business affairs, a complex and interesting area to say the least.
Bell, N., Griffin, P.C., Hoffmann, A.A. and Miller, A.D., 2018. Spatial patterns of genetic diversity among Australian alpine flora communities revealed by comparative phylogenomics. Journal of Biogeography, 45(1), pp.177-189.
Kelly has an interest in disease and the roles animals and the environment play in disease transmission. She explored this as an honours student with the PEARG Mosquito Group in early 2008 where she investigated the thermal responses of larval mosquitoes and the influence of climatic changes on their microclimates and potential range in Australia. Since then, she has spent over seven years working with PEARG on a range of projects including the fitness of Aedes aegypti mosquitoes with Wolbachia, the resistance of various wheat lines to the wheat curl mite Aceria tosichella and the wheat streak mosaic and high plains viruses of mites and wheat plants. Since October 2013, Kelly has worked on detecting and characterising Wolbachia in a diverse range of Drosophila species from around Australia. She has detected a number of interesting strains including some male-killers!
Detecting and characterising Wolbachia in a diverse range of Drosophila species
Richardson, K. M., Hoffmann, A. A., Johnson, P., Ritchie, S., Kearney, M. (2011), Thermal sensitivity of Aedes aegypti from Australia: empirical data and prediction of effects on distribution. Journal of Medical Entomology, 48:914-923
Yeap, H. L., Mee P., Walker, T., Weeks, A. R., O’Neill, S. L., Johnson, P., Ritchie, S. A., Richardson, K. M., Doig, C., Endersby, N. M., Hoffmann, A. A. (2011), Dynamics of the “Popcorn” Wolbachia infection in outbred Aedes aegypti informs prospects for mosquito vector control. Genetics, 187:583-595
Richardson, K.M., Hoffmann, A. A., Johnson, P., Ritchie, S.R. and Kearney, M.R. (2012), A replicated comparison of breeding-container suitability for the dengue vector Aedes aegypti in tropical and temperate Australia. Austral Ecology, 38:2019-229
Richardson, K., Miller, A.D., Hoffmann, A.A., Larkin, P. (2014), ‘Potential new sources of wheat curl mite resistance in wheat to prevent the spread of yield-reducing pathogens. Experimental and Applied Acarology, 64:1-19
Richardson, K.M., Schiffer, M., Griffin, P.C., Lee, S.F., Hoffmann, A.A. (2016), Tropical Drosophila pandora carry Wolbachia infections causing cytoplasmic incompatibility or male killing. Evolution, 70:1791-1802
Ross, P.A., Axford, J.K., Richardson, K.M., Endersby-Harshman, N.M., Hoffmann, A.A. (2017). Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia’. Journal of Visualized Experiments, 126:e56124
Turelli, M., Cooper, B.S., Richardson, K.M., Ginsberg, P.S., Peckenpaugh, B., Antelope, C.X., Kim, K.J., May, M.R., Abrieux, A., Wilson, D.A., Bronski, M.J., Moore, B.R., Gao, J., Eisen, M.B., Chiu, J.C., Conner, W.R., Hoffmann, A.A. (in press), Rapid global spread of wRi-like Wolbachia across multiple Drosophila. Current Biology
Australian and New Zealand Society of Comparative Physiology and Biochemistry – Sydney, 2008
2008 B.Sc (Hon) – The University of Melbourne
2008 Diploma of Arts (Environmental Studies) – The University of Melbourne
Best presentation by an Honours student – Conference of the Australian and New Zealand Society of Comparative Physiology and Biochemistry, 2008
Yang is currently working at PEARG as a population geneticist with a focus on insecticide resistance management in red legged earth mites. In the past, her research has largely focused on using techniques of molecular biology and biochemistry to understand role of ceramide-metabolism enzymes in insect development and longevity. During her PhD in Zhejiang University, China, she reported the cloning, biochemical characterization, and functional analysis of Drosophila alkaline ceramidase (Dacer), suggesting that Dacer plays an important role in the Drosophila development and longevity by controlling the metabolism of ceramides. She then worked as a research fellow in Jiangsu academy of agricultural sciences, China, characterizing ceramidase and sphingomyelinase in Chilo suppressalis. She was also involved in transcriptome analysis of Scirpophaga incertulas with focus on bet-hedging genes. From 2014, Yang shifted focus into population genetics where she explored fine-scale processes of gene flow and dispersal in resistant red legged earth mites. This knowledge can be applied to better understand the routes resistant individuals have dispersed along, that may be important in reducing the risk of resistance spreading further.
Insecticide resistance management (population genetics) in red legged earth mites
NHMRC Dengue Program
Refereed Journals (first author)
Yang Q, Sun N, Zheng M, Luo CC, Luo GH, Fang JC*，Construction of genomic ddRADseq libraries of Chilo suppressalis and Scirpophaga incertulas (Lepidoptera: Pyralidae). Nature Enemies of Insects, 2016 (6): 1114-1120.
Yang Q, Li XW, Lin XW, Zhou Y, Yuan JQ, Wang HD, Chen JA, Mao CG , Zhu ZR*, Characterization of free endogenous sphingoid bases in the golden apple snail Pomacea canaliculata: involvement in snail development and nutrient limitation. Invertebrate Reproduction & Development, 2013, 57(4): 287 – 292.
Yang Q, Zhou Y, Han GJ, Fang JC*. Isolation, identification and active ingredient analysis of two pathogenic bacterial strains from Scirpophaga incertulas (Lepidoptera: Pyralidae). Jiangsu Journal of Agricultural Science. 2012, 28(6): 1267-71.
Yang Q, Gong ZJ, Zhou Y, Yuan JQ, Cheng JA, Tian L, Li S, Lin XD, Xu RJ, Zhu ZR* Mao CG*, Role of Drosophila alkaline ceramidase (Dacer) in Drosophila development and longevity. Cellular and Molecular Life Sciences, 2010, 67(9): 1477-1490.
Refereed Journals – co-author
Zhang YL, Han YC, Liu BS, Yang Q, Guo HF, Liu ZW, Wang LH, Fang JC*, Resistance monitoring and cross-resistance role of CYP6CW1 between buprofezin and pymetrozine in field populations of Laodelphax striatellus (Fallen). Scientific Reports, 2017, 7: 14639.
Zhou Y, Lin XW, Begum MA, Zhang CH, Shi XX, Jiao WJ, Zhang YR, Yuan JQ, Li HY, Yang Q, Mao CG, Zhu ZR*, Identification and characterization of Laodelphax striatellus (Insecta: Hemiptera: Delphacidae) neutral sphingomyelinase. Insect Molecular Biology, 2017, 6(4): 392-420.
Luo GH, Yao J, Yang Q, Zhang ZC, Hoffmann AA, Fang JC*, Variability in development of the striped rice borer, Chilo suppressalis (Lepidoptera: Pyralidae), due to instar number and last instar duration. Scientific Reports, 2016, 6: a35231.
Rašic G*, Endersby-Harshman NM, Tantowijoyo W, Goundar A, White VW, Yang Q, Filipovid I, Johnson P, Hoffmann AA, Arguni E, Aedes aegypti has spatially structured and seasonally stable populations in Yogyakarta, Indonesia. Parasites and Vectors, 2015, 8: 610.
Zhou Y, Yang Q, Han GJ, Fang JC*, Isolation of genomic microsatellite molecular markers with enrichment of suppression-PCR in Scirpophaga incertulas (Lepidoptera: Pyralidae). Jiangsu Journal of Agricultural Science, 2013, 29(5): 999-1004.
Zhang YL, Guo HF, Yang Q, Li S, Wang LH, Zhang GF, and Fang JC*. Overexpression of a P450 gene (CYP6CW1) in buprofezin-resistant Laodelphax striatellus (Fallén). Pesticide Biochemistry and Physiology, 2012, 104 (3): 277-282.
Zhou Y, Lin XW, Yang Q, Zhang YR, YuanJQ, Ling XD, Xu RJ, Cheng JA, MaoCG*, Zhu ZR*. Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum. Biochimie, 2011, 93 (7): 1124-1131.
Xuefen completed her Master’s degree (Biochemistry and Molecular Biology) at Sun Yat-Sen University, China, in 2017. Her previous research focused on functional genomics, more specifically, she studied the immunity of amphioxus, aiming to understand the origin and evolution of the vertebrate immune system. Her doctoral project will mainly focus on Bryobia mites and Vegetable leafminers, which are serious crop pests in Australia. She is supervised by Ary, Paul and Peter.
Her project includes two parts: First to investigate the species complexity of Bryobia mites in broad acre crops and pastures across Australia. Since the identification of Bryobia mites are often difficult given their small size and lack of suitable morphological characters, there is little information known about their basic biology and ecology worldwide. In this part, DNA barcoding will be used in Bryobia mites to delineate and identify morphologically cryptic species which is important for implementing effective pest control strategies. The second part of the project will focus on the vegetable leaf miner (VLM), which is a polyphagous species of agromyzid fly capable of infesting vegetable and flower crops and driving severe crop losses. Since it was found in Australia in 2015, on the Cape York Peninsula, it is now at a high risk of expanding its range and causing severe detrimental impacts on Australian agriculture. For that reason, it is important to figure out the invasion route of the VLM and study its potential establishment routes, which she will use molecular, morphological and modelling data.
Marianne finished a Bachelor of General Biology at McGill University, Canada, in 2016. After coming over to Australia she completed a year of Honours under Professor Mark Elgar with the department of Zoology at Melbourne University, looking at the Australian meat ant Iridomyrmex purpureus behaviour and communication. She is currently enrolled in a Master of Philosophy with PEARG, as a part of the greater program for control, eradication and preparedness for the Vegetable Leafminer funded by Hort Innovation. The other project partners are the University of Melbourne, cesar, Plant Health Australia, AUSVEG and NAQS. She is supervised by Prof Ary Hoffmann, Dr Paul Umina and Dr Peter Ridland.
Her project will investigate the existing food web complex between host plants, leafminer fly larvae and their parasitoid wasps in Australia. This knowledge will help pinpoint local wasp species that could be applied as biological control agents in Integrated Pest Management (IPM) programs to help reduce pesticide usage. This will be valuable information to control the spread of several invasive leafminers species that are serious agricultural threats in other regions of the world and that are expected to soon reach Australian soil and crops. One of these leafminer species, Liriomyza sativae, has already been found in Queensland, at the tip of the Cape York peninsula.
Xuan Cheng, PhD candidate
Xuan completed his masters degree in Taiwan, studying the association between acetylcholinesterase and carbofuran resistance in Chilo suppressalis (striped rice stem borer). He is currently studying for his doctorate with a focus on the pest management for Halotydeus destructor (redlegged earth mite, RLEM), an invasive species introduced from South Africa into Australia. RLEM is a winter-active
mite pest, attacking a broad range of agriculturally significant plant species in Australia. Non-diapause eggs are deposited by the female in winter while diapause eggs were produced and contained in the female body. Diapause eggs can survive applications of pesticides as well as summer heat and desiccation. Currently registered pesticides can only kill mites of active stages (e.g., larvae, nymphs and adults) and hence applications of pesticides have to be carefully timed before the production of diapause eggs. Furthermore, pesticides resistance to pyrethroid and organophosphate chemicals in active mites have been found in some populations. In order to manage pesticide resistance, Xuan’s project includes three research areas:
- Understand the dominance pattern and fitness cost of L1024F substitution in the para gene in association with pyrethroid resistance.
- Investigate the mechanism of diapause induction and diapause intensity in order to improve the applications of pesticides.
- Survey the host adaptation of RLEM and the fitness cost during crop rotation, a non-chemical control option.
Publications:Cheng X., Umina P. A., and Hoffmann A. A. (2018) Influence of previous host plants on the reproductive success of a polyphagous mite pest, Halotydeus destructor (Trombidiformes: Penthaleidae). Journal of Economic Entomology. In Press (doi: 10.1093/jee/tox368)
Hill M. P., Hoffmann A. A., Umina P. A., Cheng X., Miller A. D. (2016) Genetic analysis along an invasion pathway reveals endemic cryptic taxa, but a single species with little population structure in the introduced range. Diversity and Distributions. 22: 57-72
Chang C., Cheng X., Huang X. Y., Dai. S.M. (2014) Amino acid substitutions of acetylcholinesterase associated with carbofuran resistance in Chilo suppressalis. Pest Management Science. 70: 1930-1935
Li C.X., Cheng X., and Dai S.M. (2011) Distribution and insecticide resistance of pink stem oorer, Sesamia inferens (Lepidoptera: Noctuidae), in Taiwan. Formosan Entomologist 31: 39-50
Cheng X., Chang C., and Dai S.M. (2010) Responses of striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae), from Taiwan to a range of pesticides. Pest Management Science. 66: 762–766
- Host performance of pests depends on plant types encountered in the previous generation: the case of the polyphagous redlegged earth mite pest, Halotydeus destructor. Combined Australian Entomological Society /New Zealand Entomological Society Conference in Melbourne, Australia
- The fitness cost of bifenthrin resistance of the redlegged earth mite, Halotydeus destructor. The 46th AGM & Scientific Conference of the Australian Entomological Society in Cairns, Australia
- Distribution of rice stem borers and insecticide resistance of Chilo suppressalis (Walker) in Taiwan. 27th Congress of Taiwan Entomology Society in Taipei, Taiwan,
2016 GSSA (Government Scholarship to Study Abroad) of Taiwan Ministry of Education
2013 MIFRS and MIRS of University of Melbourne
2012 Outstanding Paper Award of Taiwan Entomological Society (台灣昆蟲學會101年度最佳論文獎)
Yifan Zhang, Masters student
Yifan graduated from the Ocean University of China in 2015 and obtained a Bachelor of Biological Science (Marine Biology). After that, Yifan become a Master student of Biotechnology at the University of Melbourne. In 2017, she changed major and joined in PEARG group, focusing on the project: Costs of Wolbachia infection in mosquitoes under different rearing conditions and between nuclear-mitochondria genomes.
Yifan has two main research interests: The first is the interaction between two primary environmental factors, nutrition and temperature and how these impact on mosquitoes with Wolbachia infections. The second aspect of the project focuses on the nuclear and mitochondria incompatibility impacts the Wolbachia-infected Aedes aegypti.
Effect of environment on stability of Wolbachia in Aedes aegypti mosquitos.
Moshe obtained a Bachelor of Science from the University of Melbourne in 2016. He joined PEARG the same year to commence his Masters, focusing on the population genomics and dispersal of mosquito vectors of dengue and other arboviruses in Southeast Asia.
Moshe’s masters research has two key aspects: The first focuses on understanding the extent and impact of population structure and genetic differentiation on Aedes albopictus mosquitoes across Peninsula Malaysia. The second seeks to use estimates of genetic relatedness to tease out patterns of Aedes aegypti dispersal across an urban landscape in the city of Petaling Jaya, Selangor. Both are closely connected to Wolbachia release programs, and intended to assist in optimizing these releases over different regions and local environments.
Xinyue Gu, Visiting PhD candidate
Xinyue is a PhD candidate working with Bactrocera species and Drosophila pandora in temperature adaptation. She completed her Bachelor of Science in 2010 and became a PhD student in Sept. 2014 at China Agricultural University. Now she is visiting PEARG for a year, supported by Chinese government in the University of Melbourne. Her training and research here includes transcriptome analysis, the correlative methods and techniques of mechanistic models under climate change, and the CRISPR/CAS9 system in Drosophila will be the essential part of her doctoral dissertation.
• Wolbachia infections in D. pandora under heat stress
• The function of key genes in temperature adaptation in Bactrocera species
Gu X., Liu L., Guo S., Li Z.*, Comparative transcriptome analyses of cuticle-related genes associated with Bactrocera dorsalis(Hendel) and B. correcta(Bezzi), 1st Symposium of Tephritid Workers of Asia, Australia and Oceania (TAAO), Putrajaya, Malaysia, 2016.08.14-08.19
CAU Outstanding Cadres 2017
2nd Prize Excellent PhD Scholarship of CAU 2017/2016/2015
Excellent graduate of Beijing 2014
Samantha graduated with a first degree, Bachelor of Science Zoology, she remained at Aberystwyth University, Wales to undertake a Masters Degree in Managing the Environment, finishing in 2012. Her undergraduate thesis investigated the effects of urbanization on garden snails (Cornu aspersum Müller) and the results were later presented on BBC’s ‘Autumnwatch’. Research for her Master of Science thesis commenced in conjunction with Sustainable Forest Management Ltd and investigated the effects of plantation on ancient woodland sites (PAWS) on the abundance and diversity of arthropods.
She volunteered at the Natural History Museum in London, UK where she worked alongside Dr Andrew Polaszek, sorting through samples collected from different geographic regions and identifying parasitic Hymenoptera (particularly the superfamily Chalcidoidea). During this period, she published three papers.
Samantha’s PhD began in September 2016 at the University of Melbourne with Professor Ary Hoffmann, Dr Paul Umina and Dr Sarina Macfadyen at CSIRO acting as her supervisors. Her research fits within a larger, national Grains Research and Development Corporation (GRDC) project investigating beneficial species that can be used to control agricultural pests in grain crops.
- Parasitic Hymenoptera associated with aphids in grain crops in Australia. Monitoring D. noxia, M. persicae, other aphids, and their parasitoids in grain crops within rural Victoria.
Ward, S. E., Shih, Y. T., Ko, C-C., and Polaszek, A. (2016). Two new species of Eretmocerus Haldeman (Hymenoptera: Aphelinidae) parasitizing Aleurolobus rhododendri Takahashi and Dialeuropora decempunctata (Quaintance & Baker) (Hemiptera: Aleyrodidae) from Taiwan. Biodiversity Data Journal.
Ward, S. E., Valente, C., Gonçalves C., and Polaszek, A. (2016). Rediscovery and redescription of Centrodora damoni (Girault) (Hymenoptera: Aphelinidae) from Australia, an egg parasitoid of Gonipterus spp (Coleoptera: Curculionidae), after nearly a century. Biodiversity Data Journal.
Andrew Polaszek, A., Shih, Y-T., and Ward, S. E. (2015) A new species of Cales Howard, 1907 (Hymenoptera: Aphelinidae) parasitizing Bemisia pongamiae (Takahashi) (Hemiptera: Aleyrodidae) in Taiwan, with a key to world species of the Cales spenceri-group. Biodiversity Data Journal.
2017 Australian Entomological Society 48th AGM in Terrigal, NSW
2016 The Australian Entomological Society 47th AGM and Scientific Conference and Entomological Society of New Zealand and NIPI forum and workshop in Melbourne, Victoria (Attendee)
Oliver completed his Bachelor of Science at Monash University in 2016 (Ecology and Conservation Biology/Zoology), and during the final years of his degree, he worked as a technical assistant in an insect physiology lab at Monash’s Clayton campus. He became interested in entomology as applied to agricultural pest management following a research placement with CSIRO in 2015/2016, investigating the life history of silverleaf whitefly (Bemisia tabaci).
With PEARG, Oliver’s study focuses on earwigs as pests of grain production in Australia, developing and refining identification techniques using DNA markers. Additionally, he aims to determine some of the conditions that might induce the European earwig (Forficula auricularia) to act as a pest, beginning with the effects of environmental stressors on its feeding behaviours.
Investigating the biology and ecology of earwigs as emerging pests of Australian grain production
Eddie is a PhD candidate supervised by Dr Andrew Weeks, Dr Melissa Carew, Dr Rhys Coleman and Prof. Ary Hoffman. For the last 20 years Eddie has have been working on monitoring of freshwater ecosystems, freshwater invertebrate taxonomy and conservation.
The focus of this project is on using mitochondrial DNA ‘barcode’ to advance taxonomy of freshwater invertebrates and enable detecting different invertebrate species from a sample of water. This will bring much needed rigour to our understanding of the ecological state of freshwater ecosystems and will allow observation of changes of their biodiversity at the species level.
Gooderham, J. and Tsyrlin E (2002) “The Waterbug Book: A Photographic Guide to the Freshwater Macroinvertebrates of Temperate Australia.” CSIRO Publishing, Collingwood.
E. Tsyrlin. A key to Victorian nymphs of Leptoperla (Plecoptera: Gripopterygidae). Identification guide No 38. Cooperative Research Centre for Freshwater Ecology 2001.
E.Tsyrlin, I.C.Campbell (2002) Colonisation of wood by invertebrates in Australian upland streams: differences between pools and riffles. Verh. Internat. Verein. Limnol. 28: 1066-1069
T.J. Doeg, E. Tsyrlin, B.Van Praagh (1996) A survey for the Dandenong Freshwater Amphipod Austrogammarus australis (Sayce). Freshwater Ecology Section, Flora and Fauna Branch, Department of Natural Resources and Environment, Victoria
C.J. Walsh & Edward Tsyrlin, Michael R. Grace & Jason A. Sonneman (2002) An ecological assessment of the impact of treated effluent from Mount Hotham Alpine resort on the Dargo River and an initial assessment of Swindlers Ck.
2013 Australian Society for Limnology (ASL) annual conference.
2006 Plenary talk on Australia’s use of biological indicators at the Conference on biological indicators of River Health in StPetersburg, Russia
2005 ASL annual conference.
2002 Oral presentation at ASL annual conference.
2001 Oral presentation at ASL annual conference and International Symposium on Limnology
Eureka Book Price and Whitley medal for The Waterbug Book
Hoffmann completed Postgraduate students
2018 Perran Ross | PhD
2018 Ellen Cottingham | MsC
2018 Tom Schmidt | PhD
2017 Rahul Rane | PhD
2017 Rebecca Jordan | PhD
2017 Bryant Gagliardi | PhD
2017 Megan Hirst | PhD
2017 Peter Kriesner | PhD
2017 Peter Mee | PhD
2016 Michele Johnstone | MPhil
2016 Lihsin Wua | PhD
2015 Rhys Coleman | PhD
2015 Valentina Colombo | PhD
2015 Rachel Slatyer | PhD
2015 Katy Jeppe | PhD
2014 Heng Lin Yeap | PhD
2014 James Camac | PhD
2014 Ian Smith | PhD
2013 Kallie Townsend | PhD
2013 Jess M’Baya | PhD
2012 Purabi Ghosh | MPhil
2012 Matt Hill | PhD
2012 Elise Furlan | PhD
2011 Phillipa Griffin | PhD
2011 Sarah de Garis | MPhil
2011 Kate Mitchell | PhD
2010 Lauren Carrington | PhD
2010 Adrian Rakimov | PhD
2010 John Roberts | PhD
2010 Aston Arthur | PhD
2009 Gareth Holmes | PhD
2009 Warsito | PhD
2009 Cheeseng Chong | PhD
2008 Isabel Valenzuela | PhD
2008 Vanessa Kellermann | PhD
2008 Sean Byars | PhD
2008 Belinda Van Heerwaarden | PhD
2007 Paul Mitrovski | PhD
2007 Michael Nash | PhD
2007 David Sharley | PhD
2006 Nicole Bone | MPhil
2006 Vin Pettigrove | PhD
2006 Melissa Carew | PhD
2006 Lea Rako | PhD
2006 Marina Telonis-Scott | PhD
2005 Martina Bernard | PhD
2005 Michelle Schiffer | PhD
2005 Karen Herbert | PhD
2005 Mark Kellett | PhD
2005 Nancy Endersby | PhD
2004 Paul Umina | PhD
2004 Brad Rundle | PhD
2004 Claire Milton | PhD
2003 Angela Corrie | PhD
2003 Tracy Reynolds | PhD
2003 Andrea Magiafoglou | PhD
2003 Chantelle Sinclair | PhD
2003 Ursula Kolliker-Otte | PhD
2002 David Bennett | PhD
2001 Michelle Robinson | PhD
2000 Andrew Weeks | PhD
2000 Linda J. Thomson | PhD
1999 Nicole C. Jenkins | PhD
1999 Richard Woods | PhD
1999 Miriam Hercus | PhD
1998 Tracey Bjorksten | PhD
1998 David Clancy | PhD
1998 DeAnn C. Glenn | PhD
1997 Carla M. Sgrò | PhD
1995 Marcus Watson | MPhil
1989 Mark W. Blows | PhD
Samia conducted her PhD jointly between the University of Tunis El Manar and the University of Hawaii at Manoa in the US, sponsored by a Fulbright fellowship. She then moved to Imperial College London in the UK to do a postdoc funded by the Unesco-L’Oréal for women in science international fellowship before moving to Australia where she hold her current position with the Australian government. Dr Elfekih’s main research interests are focused on using population genomics and bioinformatics to address potential issues related to food security and global human health. She is particularly interested in the genomics of invasive insect pests and the possible effects of climate change on their distribution and invasion pathways. She is also interested in using biocontrol strategies for the management of vector-borne diseases (dengue fever, malaria) in regions where these diseases are underreported. Dr Elfekih is currently leading several international collaboration projects with various institutions such as the University of Cambridge, Imperial College London in the UK and the University of Oregon in the US. Dr Elfekih’s work was sponsored by highly-prestigious awards and fellowships such as Fulbright and EMBO, the Unesco-L’Oréal program and more recently the Royal Society UK exchange grant. Her work has been featured in the media including ELLE magazine in France, the Independent Newspaper in the UK, AL-Ahram daily in Egypt and Leaders Magazine in Tunisia.
- Arias, MB, Elfekih, S. & A.P., Vogler (2018) Population genetics and migration pathways of the Mediterranean fruit fly Ceratitis capitata inferred with coalescent methods. PeerJ
- Elfekih, S., Etter, P., Tay, W. T., Fumagalli, M., Gordon, K., Johnson, E., & De Barro, P. (2018). Genome-wide analyses of the Bemisia tabaci species complex reveal contrasting patterns of admixture and complex demographic histories. PloS one, 13(1), e0190555.
- Elfekih, S., Tay, W. T., Gordon, K., Court, L., & De Barro, P. (2017). Standardized molecular diagnostic tool for the identification of cryptic species within the Bemisia tabaciPest Management Science.
- Pearce, S. L., Clarke, D. F., East, P. D., Elfekih, S., Gordon, K. H. J., Jermiin, L. S., … & Rane, R. V. (2017). Genomic innovations, transcriptional plasticity and gene loss underlying the evolution and divergence of two highly polyphagous and invasive Helicoverpa pest species. BMC biology, 15(1), 69.
- Tay, W. T., Elfekih, S., Gordon, K. H., Delatte, H., & De Barro, P. J. (2017). The trouble with MEAM2: Implications of pseudogenes on species delimitation in the globally invasive Bemisia tabaci (Hemiptera: Aleyrodidae) cryptic species complex. Genome Biology and Evolution.
- Elfekih, S., Chen, C. Y., Hsu, J. C., Belcaid, M., & Haymer, D. (2016). Identification and preliminary characterization of chemosensory perception-associated proteins in the melon fly Bactrocera cucurbitae using RNA-seq. Scientific reports, 6.
Awards, grants and fellowships
2018 Royal Society UK mobility grant (co-applicant) (12,000.00 GBP)
2017-2018 EMBO Fellowship [7,000.00 Euros]
2013-2016 OCE postdoctoral fellowship (CSIRO) [294,942.000 AUD]
2011-2013 UNESCO-L’Oréal International Fellowship for women in science [40K$]
2015 Biochemical Society UK grant [350.00 £]
2015 Genetic Society UK training grant [1,000.00 £]
2013 Fulbright Alumni Development Grant [(2,360.63 $]
2012 Korner fellowship (University of Sussex, UK) [400£]
2011 UCLA travel grant, USA [3,300.00USD]
2011 Royal Entomological Society travel grant [500 £]
2007-2008 Fulbright fellowship
Invited talks, Seminars
- “Genome-ˇwide SNPs decipher global invasion pathways in the whitefly Bemisia tabaci species complex”. Essig Museum of Entomology (UC Berkeley). Jan 15th, 2016.
- Evolutionary genomics of Bemisia tabaci and characterization of its endosymbiont metacommunities using nextRAD sequencing. International Plant and Animal Genome Asia (July 12-ˇ15th, 2015), Singapore.
- “Evolutionary genomics of Bemisia tabaci using nextRAD sequencing". Division of
Evolution, Ecology & Genetics, Australian National University (ANU). Oct 8th, 2015.
Rujiporn is a population geneticist whose main interest is in the genetic management of rare species. She completed a Bachelor of Science with First Class Honours in 2009 at Murdoch University. Working with the Department of Biodiversity, Conservation and Attractions (Western Australia), her honours project was aimed to understand reproduction biology, mating system and pollen dispersal of Banksia nivea spp. uliginosa in the fragmented Busselton ironstone community. While she was undertaking her honours, she was also involved in a similar project for Calothamnus quadrifidus. She then worked as a Phytophthora research scientist in the same department, conducting a new phosphite application field experiment and detecting Phytophthora cinnamomic in suspected areas such as national parks.
Rujiporn developed an interest in conservation genetics and pursued a PhD at the University of Western Australia. Her PhD, which was completed in 2016, investigated genetic consequences of population mixing in translocations of burrowing bettongs and island dibblers as well as population structure and dispersal of mainland dibblers. The focuses of this project were on short- and long-term effects of admixture, factors influencing the genetic contributions of parental lineages and how population structure and dispersal patterns can be used in founder selection.
Weeks, A. R., Moro, D., Thavornkanlapachai, R., Taylor, H. R., White, N. E., Weiser, E. L., and Heinze, D. (2015). Conserving and enhancing genetic diversity in translocation programs. Pages 127-140 in D. Armstrong, M. W. Hayward, D. Moro, and P. J. Seddon, editors. Advances in reintroduction biology of Australian and New Zealand fauna. CSIRO Publishing, South Australia.
Thavornkanlapachai, R. (2014). Is mixing populations beneficial for translocation? Genetic consequences of translocating burrowing bettongs in Western Australia. Newsletter of the Australasian Wildlife Management Society 28, 8-9.
Sampson, J. F., Byrne, M., Yates, C. J., Gibson, N., Thavornkanlapachai, R., Stankowski, S., Macdonald, B., and Bennett, I. (2014). Contemporary pollen-mediated gene immigration reflects the historical isolation of a rare, animal-pollinated shrub in a fragmented landscape. Heredity 112, 172-181.
Gibson, N., Yates, C., Byrne, M., Langley, M., and Thavornkanlapachai, R. (2012). The importance of recruitment patterns versus reproductive output in the persistence of a short-range endemic shrub in a highly fragmented landscape of south-western Australia. Australian Journal of Botany 60, 643–649.
2017 12th International Mammalogical Congress, Perth, Australia
2017 Bioscience Early Career Researchers Research Summit, Melbourne, Australia
2015 Australasian Wildlife Management Society Conference, Perth, Australia
2014 Animal Biology Research Day Conference, Perth, Australia
2013 Australasian Wildlife Management Society Conference, Palmerston North, New Zealand
2017 Perth Zoo Prize for Conservation Research Award
2015 The Department of Parks and Wildlife Ad-Hoc Scholarship
2011 – 2014 Australian Postgraduate Award (APA)
2012 UWA Graduate Research School Travel Award
2009 Murdoch University Environmental Science Honours Scholarship
James Maino, Honorary Research Fellow
James researches better approaches to forecast and manage risk surrounding outbreaks of agricultural invertebrate pests. Part of this work aims to address the increasing problem of pesticide resistance in Australia, whereby a combination of resistance surveillance, novel genetic techniques, and predictive modelling are used to develop management strategies. He is interested in using the latest digital resources (e.g. satellite based climatic and land-usage data) and analysis methods that incorporate mechanistic processes to better predict pest dynamics so that impacts can be anticipated and minimised.
In 2016 James was jointly awarded a PhD at the University of Melbourne and Vrije Universiteit (Amsterdam) under the supervision of Michael Kearney and Bas Kooijman.
James is currently involved with research projects on some important species of aphids and mites, aiming to develop better management practices that utilise a variety of control strategies, in the face of increasing chemical resistance problems.James also is applying modern data sets and analysis techniques to better assess risks of biosecurity threats to agriculture, which not only includes novel species, but novel biotypes (i.e. new genes that occur overseas but not in domestic varieties).
2018 Climate contributes to the evolution of pesticide resistance JL Maino, PA Umina, AA Hoffmann Global Ecology and Biogeography 27 (2), 223-232 2018 No longer a west-side story–pesticide resistance discovered in the eastern range of a major Australian crop pest, Halotydeus destructor (Acari: Penthaleidae) JL Maino, M Binns, P Umina Crop and Pasture Science 69 (2), 216-221 2017 The universality of the von Bertalanffy growth curve. Comment on:; Physics of metabolic organization; by Marko Jusup et al. JL Maino, MR Kearney Physics of life reviews 20, 63-65 2017 The effect of egg size on hatch time and metabolic rate: theoretical and empirical insights on developing insect embryos JL Maino, EI Pirtle, MR Kearney Functional Ecology 31 (1), 227-234 2016 Mechanistic models for predicting insect responses to climate change JL Maino, JD Kong, AA Hoffmann, MG Barton, MR Kearney Current opinion in insect science 17, 81-86 2015 Ontogenetic and interspecific scaling of consumption in insects JL Maino, MR Kearney Oikos 124 (12), 1564-1570 2015 A dynamic energy budget for the whole life‐cycle of holometabolous insects AL Llandres, GM Marques, JL Maino, S Kooijman, MR Kearney, J Casas Ecological Monographs 85 (3), 353-371 2015 Testing mechanistic models of growth in insects JL Maino, MR Kearney Proc. R. Soc. B 282 (1819), 20151973 2014 Reconciling theories for metabolic scaling JL Maino, MR Kearney, RM Nisbet, SALM Kooijman Journal of Animal Ecology 83 (1), 20-29 2014 Ontogenetic and interspecific metabolic scaling in insects JL Maino, MR Kearney The American Naturalist 184 (6), 695-701
• Chancellor’s Prize for Excellence in the PhD Thesis, University of Melbourne 2016
• Student Paper of the Year Award (Runner-up), The American Naturalist 2015
• Elton Prize for best paper in the Journal of Animal Ecology by a young researcher 2014
Megan is based mainly in the National Herbarium building of the Royal Botanic Gardens Melbourne and during semester at the Burnley Campus of Melbourne University
Meg obtained a Bachelor of Applied Science-Horticulture at Burnley College whilst working on rare and threatened plants at the Royal Botanic Gardens Melbourne (RBG) under various roles (nursery technician, seed bank officer, research assistant). Meg successfully undertook an Honours year with Forest and Ecosystem Sciences under the supervision of Craig Nitschke and continued working with rare flora but with a stronger emphasis on seed conservation (working with the Victorian Conservation Seedbank, RBG). Meg undertook a PhD in ecology and evolution under the supervision of Ary Hoffmann, Neville Walsh and Philippa Griffin, and successfully completed her studies in 2017. Meg’s thesis investigated plant adaptation in a group of native Australasian daisies from the genus Brachyscome. She transplanted seeds and seedlings across different environments and in artificially heated plots to test for adaptation to local and future conditions.
Meg currently works in the Victorian Conservation Seedbank, on the Fungal Barcode Project based at the RBG and as a sessional tutor in plant morphology/identification skills at the University of Melbourne (Burnley campus). Meg is based mainly in the National Herbarium building of the Royal Botanic Gardens Melbourne, and during semester at the UniMelb Burnley Campus.
Testing the horticultural potential of rare and threatened Australian plants
Hirst, M.J. (2017). Plant evaluation and selection for conservation horticulture: improving the odds. The Botanic Garden 49, 39 -40. https://issuu.com/bganz/docs/tbg_iss49_nov17_final_171123
Hirst, M. J., Griffin, P. C., Sexton, J. P. and Hoffmann, A. A. (2017), Testing the niche-breadth–range-size hypothesis: habitat specialization vs. performance in Australian alpine daisies. Ecology 98, 2708 – 2724
Messina, A., Hirst, M.J., Walsh, N.G. (2017) Getting by with a little help from my friends. Samara: The International Newsletter of the Millennium Seed Bank Partnership. Issue 30. 13
Hirst, M. J., Sexton, J. P., and Hoffmann, A. A. (2016) Extensive variation, but not local adaptation in an Australian alpine daisy. Ecology and Evolution 6, 5459 – 5472
Hirst, M.J. (2013). A global message from an old bird. The Botanic Garden 37, 9–10. http://issuu.com/bganz/docs/tbg_iss37_dec2013/2
Slatyer, R.A., Hirst, M. and J.P. Sexton. (2013). Niche breadth predicts geographic range size: a general ecological pattern. Ecology Letters. doi:10.111/ele.12140.
Hirst, M.J. (2013). A phylogenetic and morphological approach in a key Australian plant genus, Brachyscome. Australasian Plant Conservation 21, 24–26.
Hirst, M.J. (2012). Native daisies add versatility. Australian Horticulture 109, 8.
2018 – 2019: Helen McLellan Research Grant, Royal Botanic Gardens Victoria:
2018 – 2019: Australian Flora Foundation Research Grant
2018: Botanic Gardens of Australia and New Zealand Professional Development Award.
I am a master student from Berlin/Germany and joined the Hoffmann lab for 6 months to complete an internship as part of my masters program at the Freie Universität Berlin. I obtained my bachelor degree in biology 2016 and worked with a variety of insects, including flies, wasps, termites and different species of beetles. During my final bachelor project, supervised by Prof. Jens Rolff, I investigated antimicrobial peptides in the immune system of T. molitor respectively the ability of bacteria to evolve resistances against these substances. Further I also studied immune responses of T. molitor during infections with the entomopathogenic fungus B. bassiana. During my stay with PEARG I gained skills in the maintenance of Mosquito colonies, artificial blood feeding methods as well as DNA analyses.
I am currently working on the impact of non-human blood types (e.g pig and sheep) on Ae. aegypti and its Wolbachia infection.
Maistrou, S., Paris, V., Jensen, A.B., Rolff, J., Meyling, N.V., Zanchi, C. (in prep): A constitutively expressed antifungal peptide protects Tenebrio molitor during a natural infection by the entomopathogenic fungus Beauveria bassiana.
- 2017: PROMOS scholarship (Program for the mobility of undergraduate and postgraduate students)
- Funding of internships outside Europe
- 2017: PROMOS scholarship (Program for the mobility of undergraduate and postgraduate students)
Weibin is an associate professor at Shanghai Normal University and has joined PEARG as a visiting scholar in 2017. Weibin was awarded a PhD in Molecular Ecology at East China Normal University under the supervision of Professor Xiaoming Wang, joint curator of Shanghai Science & Technology Museum, Shanghai Natural History Museum and Shanghai Planetarium. His research focus is on the infection rate and effect of Wolbachia in natural populations of Lepidoptera insects.
Study on the influence of mitochondrial variation in genus Polytremis caused by Wolbachia infection
Jiang WB (2017) Molecular phylogeny and taxonomy of lepidoptera with special reference to influence of Wolbachia Infection in the Genus Polytremis. Chapter 3 of Lepidoptera, 42-62, InTech Press, ISBN 978-953-51-3660-6.
Jiang WB, He HY, Li YD, Ren MY, Ma YZ, Zheng LL, Zhu JQ, Yu WD* (2016) Taxonomic status and molecular phylogeography of two sibling species of Polytremis (Lepidoptera: Hesperiidae). Scientific Reports, 6: 20820.
Jiang WB, Chen MH, He HY, Wu YJ, Zhu JQ, Yu WD* (2016) Eleven microsatellite loci developed from the Chinese Hesperid butterflies, Polytremis fukia (Lepidoptera: Hesperiidae), and cross-amplification in other species of Polytremis. Applied Entomology and Zoology, 51(4): 669–672.
Jiang WB, Zhu JQ*, Yang QC, Zhao HD, Chen MH, He HY, Yu WD* (2016) Complete mitochondrial DNA genome of Polytremis nascens (Lepidoptera: Hesperiidae). Mitochondrial DNA Part A, 27(5), 3131-3132.
Jiang WB, Zhu JQ, Chen MH, Yang QC, Du X, Chen SY, Zhang LN, Yu YM, Yu WD* (2014) Wolbachia infection status and genetic structure in natural populations of Polytremis nascens (Lepidoptera: Hesperiidae). Infection, Genetics and Evolution, 27: 202-211.
Jiang WB, Zhu JQ*, Zhan L, Chen MH, Song C, Yu WD* (2014) Isolation and characterization of microsatellite loci in Polytremis nascens (Lepidoptera: Hesperiidae), and their cross-amplification in related species. Applied Entomology and Zoology, 49: 177-181.
Jiang WB, Zhu JQ*, Song C, Li XY, Yang Y, Yu WD* (2013) Molecular phylogeny of the butterfly genus Polytremis (Hesperiidae, Hesperiinae, Baorini) in China. PLoS ONE, 8 (12): e84098.
Jiang WB, Liu N, Zhang GT, Renqing PC, Xie F, Li TY, Wang ZH*, Wang XM* (2012) Specific detection of Echinococcus spp. from the Tibetan fox (Vulpes ferrilata) and the red fox (V. vulpes) using copro-DNA PCR analysis, Parasitology Research, 111: 1531-1539.
Jiang WB, Wang XM, Li M, Wang ZH* (2011) Identification of the Tibetan fox (Vulpes ferrilata) and the red fox (Vulpes vulpes) by copro-DNA diagnosis. Molecular Ecology Resources, 11 (1): 206-210.
Hong Y, Peng JB, Jiang WB, Lin JJ* (2011) Proteomic analysis of Schistosoma japonicum schistosomulum proteins that are differentially expressed among hosts differing in their susceptibility to the infection, Molecular & Cellular Proteomics, 10(8): M110.006098.
Peng JB, Han HX, Gobert GN, Hong Y, Jiang WB, Lin JJ* (2011) Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB/c mice, Parasites & Vectors, 4: 155.
Jiang WB, Hong Y, Peng JB, Fu ZQ, Feng XG, Liu JM, Shi YJ, Lin JJ* (2010) Study on Differences in the Pathology, T Cell Subsets and Gene Expression in Susceptible and Non-Susceptible Hosts Infected with Schistosoma japonicum, PLoS ONE, 5 (10): e13494.
Li-Jun is a research fellow in the Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences. He completed his Ph.D. studies at Beijing Forestry University in 2016. His studies focused on the origin and dispersal of several pest insects. This January he joined PEARG as a visiting scholar investigating interactions between Wolbachia and viruses. He will detect Wolbachia strains in field populations of Drosophila simulans and investigate the abundance of viruses in Wolbachia positive and negative populations from different locations.
I received my PhD many years ago at the University of Texas Southwestern Medical School with a Drosophila evolutionary geneticist, Costas Kastritsis who had studied inversion polymorphisms with T. Dobzhansky. I then did a post doc at the University of Texas, Austin, studying ecdysone and chromosome activity in Rhynchosciara, particularly in relation to gene amplification. My supervisor was the Brazilian geneticist, Crodowaldo Pavan. After that I came to Melbourne and worked for several years in the Melbourne University Genetics Department at the end of the time of Michael White’s tenure there. I made the mistake of trying to raise Rhynchosciara in Australia since I was very familiar with that fly and I felt that there was a lot of work still to be done. That attempt was not successful. Subsequently, I had a long period at the University of Sao Paulo, Brazil working with Professor Francisco Lara. I have been in and out of Brazil for many years through various political changes and still visit there. During some of that period I was a lecturer for about 8 years at the College of Advanced Education which became the Department of Education of the University of Melbourne. I began to work with Ary when I came back from a long period in Brazil and he was still at La Trobe University. I could have retired but I decided to work a little longer. My first project involved clines in inversion polymorphism. I have been associated with Ary’s group ever since, working casually on various projects and most recently in an honorary position. My speciality is chromosomes, particularly fly chromosomes, which most people do not work with anymore.
Chromosome evolution in Australian Scaptodrosophila: I have examined mitotic chromosomes of a number of Australian Scaptodrosophila species and carried out C-banding and Ag-NOR banding. I have also examined polytene chromosomes. Since Ag-NOR banding was only variably successful, I may also do rDNA in situ hybridization when I am in Brazil. I intend to put together my results in a manuscript showing the relationship among the species.
- Stocker AJ, Foley B, Hoffmann AA (2004) Inversion frequencies along a latitudinal cline in Australian populations of Drosophila serrata. Genome Dec; 47(6) 1144-53.
- Stocker AJ, Madalena CG, Gorab E (2006) The effects of temperature shock on transcription and replication in Rhynchosciara americana (Diptera: Sciaridae). Genetica Mar 126(3) 277-90.
- Fiorini A, Gouveia FS, Soares MAM, Stocker AJ, Ciferri RR, Fernandez MA (2006) DNA bending in the replication zone of the C3 DNA puff amplicon of Rhynchosciara americana (Diptera: Sciaridae). Mol Biol Rep. Mar 33(1) 71-82.
- Rako L, Anderson AR, Sgro CM, Stocker AJ, Hoffmann AA (2006) The association between inversion In(3R)Payne and clinally varying traits in Drosophila melanogaster. Genetica Sep-Nov 128(1-3) 373-84.
- Madalena CG, Amabis JM, Stocker AJ, Gorab E (2006) The localization of ribosomal DNA in Sciaridae (Diptera: Nematocera) reassessed. Chromosome Res (2007) 15(4) 409-16.
- Gorab E, Amabis JM, Stocker AJ, Drummond L and Stollar BD (2009) Potential sites of triple-helical nucleic acid formation in chromosomes of Rhynchosciara (Diptera: Sciaridae) and Drosophila melanogaster. Chromosome Res. 17(6) 821-32.
- Stocker AJ, Rusuwa BB, Blacket MJ, Frentiu FD, Sullivan M, Foley BR, Beatson S, Hoffmann AA, Chenoweth SF (2012) Physical and linkage maps for Drosophila serrata, a model species for studies of clinal adaptation and sexual selection. G3 (Bethesda) 2(2) 287-297.
- Soares MAM, Hartfelder K, Tesserolli de Souza JM, Stocker AJ (2015) Developental ecdysteroid titers and DNA puffs in larvae of two sciarid species, Rhynchosciara americana and R. milleri (Diptera: Sciaridae). Genetica 143(5) 597-612.