Perran has been a member of the Hoffmann lab since 2012, where he previously completed a MSc and PhD working with Aedes aegypti mosquitoes. He is interested in disease control programs that deploy modified insects into natural populations and the factors that could affect their success. During his PhD, he investigated the effects of different environmental conditions on Wolbachia infections and the mosquitoes they infect. His current research will involve generating new Wolbachia infections in Aedes aegypti and insect pests to provide more options for pest and disease vector control.
- Generating new Wolbachia infections for pest and disease vector control
- Laboratory adaptation and inbreeding in mosquitoes
- Wolbachia infections under heat stress
- Ross, P. A., Axford, J. K., Richardson, K. M., Endersby-Harshman, N. M., & Hoffmann, A. A. (2017). Maintaining Aedes aegypti mosquitoes infected with Wolbachia. Journal of visualized experiments. In press.
- Ross, P. A., Wiwatanaratanabutr, I., Axford, J. K., White, V. L., Endersby-Harshman, N. M., & Hoffmann, A. A. (2017). Wolbachia infections in Aedes aegypti differ markedly in their response to cyclical heat stress. PLoS pathogens, 13(1), e1006006.
- Ross, P. A., Endersby, N. M., & Hoffmann, A. A. (2016). Costs of three Wolbachia infections on the survival of Aedes aegypti larvae under starvation conditions. PLoS neglected tropical diseases, 10(1).
- Axford, J. K., Ross, P. A., Yeap, H. L., Callahan, A. G., & Hoffmann, A. A. (2015). Fitness of wAlbB Wolbachia infection in Aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion. The American journal of tropical medicine and hygiene, 15-0608.
- Hoffmann, A. A., Ross, P. A., & Rašić, G. (2015). Wolbachia strains for disease control: ecological and evolutionary considerations. Evolutionary applications 8(8), 751-768.
- Ross, P. A., Endersby, N. M., Yeap, H. L., & Hoffmann, A. A. (2014). Larval competition extends developmental time and decreases adult size of wMelPop Wolbachia-infected Aedes aegypti. The American journal of tropical medicine and hygiene, 91(1), 198-205.
Genetics Society of Australasia 2014 “Larval competition reduces the fitness of Wolbachia-infected Aedes aegypti”
American Society of Tropical Medicine and Hygiene 2015 “Wolbachia infection reduces the starvation resistance of Aedes aegypti larvae”
Mosquito Control Association of Australia 2016 “Wolbachia infections in Aedes aegypti are adversely affected by cyclical heat stress”
Australian Fly Meeting 2016 “Environmental impacts on the use of Wolbachia for arbovirus control”
IMPACT7 2017 “Using bacteria to fight mosquito-borne disease”
Royal Society of Victoria Young Scientist Research Prize (Finalist)
Australian Government Research Training Program Scholarship
School of BioSciences Travel Scholarship
Bio21 Travel Scholarship
Personal website: perranross.wordpress.com
Google Scholar: goo.gl/WZH0Jp
Nancy Endersby-Harshman, Research Fellow and Laboratory manager
Nancy is a Research Fellow in the Pest and Environmental Adaptation Research Group in the Hoffmann Laboratory, Bio21 Institute, School of BioSciences at the University of Melbourne. Nancy has a background in horticultural entomology and a PhD from Monash University on the genetic structure of Australian populations of the horticultural pest, the diamondback moth, Plutella xylostella. Nancy currently conducts research on the dengue vector mosquito, Aedes aegypti, in the fields of insecticide resistance, population genetics, Wolbachia pipientis and vector ecology. Nancy manages the group’s Molecular Laboratory and the Arboviral Research Laboratory, a Biosecurity Approved Arrangement with the Department of Agriculture and Water Resources.
Our laboratory studies release of Wolbachia-infected mosquitoes (Aedes aegypti) to reduce the impact of dengue virus. Wolbachia-infected mosquitoes do not transmit dengue and we aim to replace the natural population of the dengue vector with the same mosquito species in which virus replication is blocked. The Wolbachia strategy relies on the released mosquitoes being fit and competitive in the field and different strains of the Wolbachia bacterium may have differing fitness costs to the mosquito. So, an important part of our research is to do with maintaining a high level of quality in our mosquitoes for release.
After Wolbachia-infected mosquitoes were released with success in Cairns, the program was mobilised with releases occurring in parts of Asia and South America where different levels of success in establishment of the Wolbachia mosquito lines have been experienced. Consequently, we now investigate environmental factors which may act as barriers to establishment of Wolbachia strains in the environment in an effort to find strains that will perform robustly in the field.
Current projects in this field look at
- effect of environmental levels of antibiotic contamination on Wolbachia-infected mosquitoes
- background levels of insecticide resistance in Aedes aegypti at potential release sites with a particular focus on pyrethroid insecticides and target site resistance
- Other insecticide resistance projects include assessment of resistance status of Ae. aegypti which arrive in Australia as exotic incursions in comparison with the local population of Ae. aegypti mosquitoes in Queensland.
Outbreaks of dengue fever have occurred in Queensland, Australia, since the late 1800s, leading to ongoing attempts to control the mosquito vector, Aedes aegypti (L.). Since the 1990s, pyrethroid insecticides have been used for this purpose, but have been applied in a strategic manner with a variety of delivery methods including indoor residual spraying, lethal ovitraps and use of insect growth regulators as larvicides. Separate selection experiments that we conducted on mosquitoes from Queensland, using Type I and Type II pyrethroids did not produce resistant lines of Ae. aegypti and bioassays of field material from Queensland showed only weak tolerance in comparison with a susceptible line. There was no evidence of knockdown resistance (kdr) mutations in Ae. aegypti from Queensland, in stark contrast to the situation in nearby southeast Asia. We suspect that careful management of pyrethroid insecticide use combined with surveillance and interception of exotic incursions has helped to maintain pyrethroid (and particularly kdr – based) susceptibility in Ae. aegypti in Australia.
Refereed Journals (first, joint first or senior author)
Endersby-Harshman, NM, Wuliandari JR, Harshman LG, Frohn V, Johnson BJ, Ritchie SA, Hoffmann AA. 2017. Pyrethroid susceptibility has been maintained in the dengue vector, Aedes aegypti (Diptera: Culicidae), in Queensland, Australia. Journal of Medical Entomology 54: 1649-1658.
Rašić G*, Endersby-Harshman NM*, Tantowijoyo W, Goundar A, White VL, Yang Q, Filipović I, Johnson P, Hoffmann AA, Arguni E (2015) Aedes aegypti has spatially structured and seasonally stable populations in Yogyakarta, Indonesia. Parasites & Vectors 12/2015; 8(1). DOI:10.1186/s13071-015-1230-6 *Joint first authors.
Rochmijati Wuliandari J, Lee SF, White VL, Tantowijoyo W, Hoffmann AA, Endersby-Harshman NM (2015). Association between three mutations, F1565C, V1023G and S996P, in the voltage-sensitive sodium channel gene and knockdown resistance in Aedes aegypti from Yogyakarta, Indonesia. Wang C, ed. Insects. 2015; 6 (3): 658-685. doi:10.3390/insects6030658.
Endersby NM, White VL, Chan J, Hurst T, Rašić G, Miller A, Hoffmann AA (2013). Evidence of cryptic genetic lineages within Aedes notoscriptus (Skuse). Infection, Genetics and Evolution 18: 191-201.
Endersby NM & Hoffmann AA (2013). Effect of Wolbachia on insecticide susceptibility in lines of Aedes aegypti. Bulletin of Entomological Research 103 (3), 269-277.
Endersby NM, Hoffmann AA, White VL, Ritchie S, Johnson PH, Rapley LP, Weeks AR (2011). Changes in the genetic structure of Aedes aegypti (Diptera: Culicidae) populations in Queensland, Australia, across two seasons: Implications for potential mosquito releases. Journal of Medical Entomology 48 (5): 999-1007.
Endersby NM, Viduka K, Baxter SW, Saw J, Heckel DG & McKechnie SW (2011). Widespread pyrethroid resistance in Australian diamondback moth, Plutella xylostella (L.), is related to multiple mutations in the para sodium channel gene. Bulletin of Entomological Research 101: 393–405.
Endersby NM, Hoffmann AA, White VL, Lowenstein S, Ritchie S, Johnson PH, Rapley LP, Ryan PA, Nam VS, Yen NT, Kittiyapong P, Weeks AR (2009). Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and Exon Primed Intron Crossing markers suggests feasibility of local control options. Journal of Medical Entomology 46 (5): 1074-1083.
Endersby NM, Ridland PM and Hoffmann AA (2008). The effects of local selection versus dispersal on insecticide resistance patterns: longitudinal evidence from diamondback moth (Plutella xylostella (Lepidoptera: Plutellidae)) in Australia evolving resistance to pyrethroids. Bulletin of Entomological Research 98: 145 – 157.
Endersby NM, Hoffmann AA, McKechnie SW and Weeks AR (2007). Is there genetic structure in populations of Helicoverpa armigera in Australia? Entomologia Experimentalis et Applicata 122: 253-263.
Endersby NM, McKechnie SW, Ridland PM and Weeks AR (2006). Microsatellites reveal a lack of structure in Australian populations of the diamondback moth, Plutella xylostella (L.). Molecular Ecology 15: 107-118.
Endersby NM, McKechnie SW, Vogel H, Gahan LJ, Baxter SW, Ridland PM and Weeks AR (2005). Microsatellites isolated from diamondback moth, Plutella xylostella (L.), for studies of dispersal in Australian populations. Molecular Ecology Notes 5: 51-53.
Endersby NM, Morgan WC, Stevenson BC and Waters CT (1992). Alternatives to regular insecticide applications for control of lepidopterous pests of Brassica oleracea var. capitata. Biological Agriculture and Horticulture 8: 189-203.
Endersby NM and Morgan WC (1991). Alternatives to synthetic chemical insecticides for use in crucifer crops. Biological Agriculture and Horticulture 8: 33-52.
Endersby NM, New TR and Thornton IWB (1990). Psocoptera from the Grampians and Mt. Arapiles, western Victoria – a biogeographic analysis. Journal of the Australian Entomological Society 29: 215-224.
Refereed Journals – co-author
Arnold, A., A. Kodym, N. M. Endersby-Harshman, J. Delpratt, and A. A. Hoffmann. 2017. Genetic structure of Gahnia radula (Cyperaceae), a key sedge for revegetation. Australian Journal of Botany 65: 128-139.
Ross, P. A., J. K. Axford, K. M. Richardson, N. M. Endersby-Harshman, and A. A. Hoffmann. 2017. Maintaining Aedes aegypti mosquitoes infected with Wolbachia. Journal of visualized experiments: JoVE.
Ross, P.A., I. Wiwatanaratanabutr, J.K. Axford, V.L. White, N.M. Endersby-Harshman, and A.A. Hoffmann, 2017, Wolbachia infections in Aedes aegypti differ markedly in their response to cyclical heat stress. PLOS Pathogens, 13 (1): p. e1006006.
Ross, P., N.M. Endersby, and A.A. Hoffmann, 2016, Costs of three Wolbachia infections on the survival of Aedes aegypti larvae under starvation conditions. PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004320 (2016), (1): p. e0004320.
H L Yeap, G Rašić, N M Endersby-Harshman, S F Lee, E Arguni, H Le Nguyen, A A Hoffmann: Mitochondrial DNA variants help monitor the dynamics of Wolbachia invasion into host populations. Heredity 11/2015; DOI:10.1038/hdy.2015.97
Gordana Rasic, Renata Schama, Rosanna Powell, Rafael Maciel-de Freitas, Nancy M. Endersby-Harshman, Igor Filipović, Gabriel Sylvestre, Renato C. Máspero, Ary A. Hoffmann: Contrasting genetic structure between mitochondrial and nuclear markers in the dengue fever mosquito from Rio de Janeiro: Implications for vector control. Evolutionary Applications 08/2015; 8(9). DOI:10.1111/eva.12301
Ross, PA, Endersby, N. M., Yeap, H. L., & Hoffmann, A. A. (2014). Larval competition extends developmental time and decreases adult size of wMelPop Wolbachia-infected Aedes aegypti. American Journal of Tropical Medicine and Hygiene, 91(1), 198-205.
Rasic, G., Endersby NM, Williams C, & Hoffmann AA (2014). Using Wolbachia-based release for suppression of Aedes mosquitoes: insights from genetic data and population simulations. Ecological applications, 24 (5), 1226-1234.
Yeap HL, Axford JK, Popovici J, Endersby NM, Iturbe-Ormaetxe I, Ritchie SA, . . . Hoffmann AA (2014). Assessing quality of life-shortening Wolbachia-infected Aedes aegypti mosquitoes in the field based on capture rates and morphometric assessments. Parasites & Vectors 7, 13 pages. doi:10.1186/1756-305-7-58.
White VL, Endersby NM, Chan J, Hoffmann AA, & Weeks AR (2014). Developing Exon-Primed Intron-Crossing (EPIC) markers for population genetic studies in three Aedes disease vectors. Insect Science. doi:10.1111/1744-7917.12145.
Díaz F, Endersby NM & Hoffmann AA (2014). Genetic structure of the whitefly Bemisia tabaci populations in Colombia following a recent invasion. Insect Science. doi:10.1111/1744-7917.12129.
Olanratmanee P, Kittayapong P, Chansang C, Hoffmann AA, Weeks AR & Endersby NM 2013. Population genetic structure of Aedes (Stegomyia) aegypti (L.) at a micro-spatial scale in Thailand: implications for a dengue suppression strategy. PLoS Neglected Tropical Diseases 7, e1913.
Lee SF, White VL, Weeks AR, Hoffmann AA & Endersby NM (2012). High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans. Applied and Environmental Microbiology 78, 4740-4743.
Yeap HL, Mee P, Walker T, Weeks AR, O’Neill SL, Johnson P, Ritchie SA, Richardson KM, Doig C, Endersby NM, Hoffmann AA (2011). Dynamics of the ‘popcorn’ Wolbachia infection in outbred Aedes aegypti informs prospects for mosquito vector control. Genetics 187: 583-595.
Weeks AR, Endersby NM, Lange CL, Lowe A, Zalucki MP & Hoffmann AA (2010). Genetic variation among Helicoverpa armigera populations as assessed by microsatellites: a cautionary tale about accurate allele scoring. Bulletin of Entomological Research 100: 445–450.
Shelton AM, Gujar GT, Chen M, Rauf A, Srinivasan R, Kalia V, Mittal A, Kumari A, Ramesh K, Borkakatti R, Zhao JZ, Endersby N, Russell D, Wu YD & Uijtewaal B. (2009). Assessing the susceptibility of cruciferous Lepidoptera to Cry1Ba2 and Cry1Ca4 for future transgenic cruciferous vegetables. Journal of Economic Entomology 102 (6): 2217-2223.
Furlong MJ, Spafford H, Ridland PM, Endersby NM, Edwards OR, Baker GJ, Keller MA, Paull CA (2008). Ecology of diamondback moth in Australian canola: landscape perspectives and the implications for management. Australian Journal of Experimental Agriculture 48: 1494–1505.
Hamilton AJ, Endersby NM, Schellhorn NA, Ridland PM, Rogers PM, Jevremov D and Baker G (2006). Evaluation of fixed sample-size plans for Plutella xylostella (Lepidoptera: Plutellidae) on broccoli crops in Australia. Journal of Economic Entomology 99: 2171-2176.
Saw J, Endersby NM and McKechnie SW (2006). Low mtDNA diversity among widespread Australian diamondback moth Plutella xylostella (L.) suggests isolation and a founder effect. Insect Science 13: 237-241.
Hamilton AJ, Endersby NM, Ridland PM, Zhang J and Neal M (2005). Effects of cultivar on oviposition preference, larval feeding and development time of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), on some Brassica oleracea vegetables in Victoria. Australian Journal of Entomology 44: 284-287.
Hamilton AJ, Versace V, Hepworth G, Stagnitti F, Dawson J, Ridland PM, Endersby NM, Schellhorn NA, Mansfield C and Rogers PM (2005). Attending to risk in sequential sampling plans. In Environmental Health Risk III, C.A. Brebbia, V. Popov, & D. Fayzieva (eds.). The Sustainable World series, vol. 13. Wessex Institute of Technology. pp. 11-20.
Hamilton AJ, Schellhorn NA, Ridland PM, Endersby NM and Ward SA (2004). A dynamic binomial sequential sampling plan for diamondback moth, Plutella xylostella. Journal of Economic Entomology 96: 127-135.
Vickers R, Ridland PM and Endersby NM (2001). Australia leads the way in the fight against the diamondback moth. Pesticide Outlook 12: 185-187.
Conference Proceedings (Editor)
Endersby NM & Ridland PM eds (2004). The management of diamondback moth and other crucifer pests: Proceedings of the 4th International Workshop, Melbourne, Australia, 26-29 November 2001.
Endersby-Harshman NM (2016) From our special correspondent… The journalism of Arthur Ransome in Egypt. Amazon Publications, Kendal, Cumbria, United Kingdom, 310 pp.
Donald C, Endersby NM, Ridland PM, Porter I and Lawrence J (2000). Field guide to pests, diseases and disorders of vegetable brassicas. Department of Natural Resources and Environment, Victoria, Australia.
Andrew Hamilton, Vincent Versace, Graham Hepworth, Frank Stagnitti, Joanne Dawson, Peter M Ridland, Nancy M Endersby: Attending to risk in sequential sampling plans. Environmental Health Risk III, Edited by C.A. Brebbia, V. Popov, D. Fayzieva, 09/2005: pages 11-20; Wessex Institute of Technology., ISBN: ISBN: 1-84564-026-8
Full papers in Proceedings
Endersby NM (1991). Alternatives to synthetic insecticides: control of cabbage white butterfly and diamondback moth. Proceedings 1st National Conference Australian Society of Horticultural Science. Sustainable management of pests, diseases and weeds. Australian Horticulture Clean and Green in the 90’s. Macquarie University, Sydney, September 30 – October 3 1991, pp. 301-305.
Endersby NM (1991). Reduced use of synthetic insecticides: control of cabbage white butterfly and diamondback moth. Proceedings 1st National Conference Australian Society of Horticultural Science. Sustainable management of pests, diseases and weeds. Australian Horticulture Clean and Green in the 90’s. Macquarie University, Sydney, September 30 – October 3 1991, pp. 385-389.
Endersby NM (1992). Development of action thresholds for Pieris rapae (Lepidoptera: Pieridae) and Plutella xylostella (Lepidoptera: Yponomeutidae). Proceedings of the 5th Australian Applied Entomological Research Conference, Pest Control and Sustainable Agriculture, 27 April – 1 May 1992, Canberra, pp. 148-151.
Endersby NM and Ridland PM (1997). Insecticide resistance in diamondback moth, Plutella xylostella (L.) in southern Australia. In: The Management of Diamondback Moth and other Crucifer Pests: Proceedings of the Third International Workshop (eds A Sivapragasam, WH Loke, AK Hussan & GS Lim). Malaysian Agricultural Research and Development Institute, Kuala Lumpur, Malaysia, pp. 290-294.
Endersby NM, Ridland PM and Zhang J. (2004). Reduced susceptibility to permethrin in diamondback moth populations from vegetable and non-vegetable hosts in southern Australia. In: The management of diamondback moth and other crucifer pests: Proceedings of the Fourth International Workshop (eds NM Endersby & PM Ridland), 26 – 29 November 2001, Melbourne, Australia, pp. 319-325.
Endersby NM and Cameron PJ (2004). Parasitism of Nyctemera amica (White) (Lepidoptera: Arctiidae) and Plutella xylostella (L.) (Lepidoptera: Plutellidae) by Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae). In: The management of diamondback moth and other crucifer pests: Proceedings of the Fourth International Workshop (eds NM Endersby & PM Ridland), 26 – 29 November 2001, Melbourne, Australia, pp. 265-268.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2003). Development of genetic markers to study dispersal of diamondback moth, Plutella xylostella (L.) in Australia. In: Proceedings of the 13th Biennial Australian Research Assembly on Brassicas, Tamworth NSW, 8-12 Sep-2003, pp. 58-61.
Endersby NM, McKechnie SW and Ridland PM (2004). Population structure and movement of diamondback moth in Australia: beginnings of a molecular marker approach. In: Improving Biocontrol of Plutella xylostella. Proceedings of the International symposium (eds AA Kirk and D Bordat), CIRAD, Montpellier, France, 21-24 October 2002, pp. 167-171.
Endersby NM (2008). Population structure and gene flow in diamondback moth in Australia and around the world – current state of knowledge and directions for the future. In: Management of Diamondback Moth and Other Crucifer Pests: Proceedings of the Fifth International Workshop (eds AM Shelton, HL Collins,Y Zhang and Q Wu), Beijing China, October 24-27, 2006, China Agricultural Science and Technology Press, pp. 132-147.
Ridland PM and Endersby NM (2008). Diamondback Moth: Messages from a land down under. In: Management of Diamondback Moth and Other Crucifer Pests: Proceedings of the Fifth International Workshop (eds AM Shelton, HL Collins,Y Zhang and Q Wu), Beijing China, October 24-27, 2006, China Agricultural Science and Technology Press, pp. 1-29.
Ridland PM and Endersby NM (2008). Seasonal phenology of diamondback moth populations in southern Australia. In: Management of Diamondback Moth and Other Crucifer Pests: Proceedings of the Fifth International Workshop (eds AM Shelton, HL Collins,Y Zhang and Q Wu), Beijing China, October 24-27, 2006, China Agricultural Science and Technology Press, pp. 90-101.
Ridland PM and Endersby NM (2011). Some Australian populations of diamondback moth, Plutella xylostella (L.) show reduced susceptibility to fipronil. In: Proceedings of the Sixth International Workshop on Management of Diamondback Moth and Other Crucifer Pests (eds ), AVRDC World Vegetable Center, pp. 207-215.
Endersby NM & Ridland PM (1994). Insecticide resistance in Victorian populations of diamondback moth, Plutella xylostella (L.). Australian Entomological Society 25th Annual General Meeting and Scientific Conference. The University of Adelaide, 24-28 September 1994.
Endersby N, Ridland P, Heisswolf S and Houlding B (1996). Plutella – A resistant pest. Vegtec 2000. 1996 National Vegetable & Potato Industry Conference (AUSVEG), Brisbane Convention Centre, 7-9 July 1996.
Endersby NM, Ridland PM and Zhang J (2000) Resistance to permethrin in diamondback moth populations from vegetable and non-vegetable hosts in southern Australia.
Australian Entomological Society 31st AGM & Scientific Conference, Northern Territory University, Darwin, 25 – 30 June 2000.
Endersby NM, Ridland PM and Zhang J (2000) Resistance to permethrin in diamondback moth populations from vegetable and non-vegetable hosts in southern Australia. NRE Horticulture Conference, Institute for Horticultural Development, 6-7 September 2000.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2002). Population structure and movement of diamondback moth in Australia: beginnings of a molecular marker approach Australian Entomological Society 33rd AGM & Scientific Conference, Fremantle WA, 22-27-Sep-2002.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2003). Population structure and movement of diamondback moth in Australia: beginnings of a molecular marker approach. Department of Primary Industries and Department of Sustainability & Environment: Entomology Symposium. Rutherglen, May 7–8, 2003.
Endersby NM, Weeks AR, McKechnie SW and Ridland PM (2003). Development of microsatellites to study population structure and movement of diamondback moth, Plutella xylostella (L.) in Australia, Australian Entomological Society 34th AGM & Scientific Conference, Hobart, Tasmania, 28 Sep-3 Oct –2003.
Endersby NM, McKechnie SW, Ridland PM and Weeks AR (2004). Population structure of Plutella xylostella (L.) investigated using microsatellite markers. XXII International Congress of Entomology, Brisbane, Queensland, Australia, 15-21 August 2004.
Endersby NM, White VL, Chan J, Hurst TP, Rašić G, Miller AD and Hoffmann AA (2012). Molecular markers provide evidence of cryptic species in Aedes notoscriptus. 11th Arbovirus Research In Australia. 10th Mosquito Control Association of Australia Symposium September 9 – 14, 2012. Outrigger Surfers Paradise, Surfers Paradise QLD, Australia.
Selection of Industry Publications
Endersby NM (1989). Cleaner Agriculture. Rural Quarterly, Spring 1989, Vol. 2, No. 3, p.18.
Endersby NM (1990). Companion Planting with insect repelling herbs. Crop Protection Bulletin, August 1990, No. 14, p. 22 & Geelong Advertiser, Tuesday 25 September, 1990.
Endersby NM (1990). Reduced synthetic insecticide inputs in vegetable growing. Crop Protection Bulletin, March 1990, No. 14, p. 14.
Endersby NM (1991). Reducing insecticide use in brassicas. Crop Protection Bulletin, September 1991, No. 26, p. 15.
Endersby NM (1991). Reduced use of synthetic insecticides: control of cabbage white butterfly and diamondback moth. Victorian Entomologist 21 (5): 127 – 129.
Endersby NM (1992). Entomopathogenic fungi. Natural biological control of brassica pests. Crop Protection Bulletin, September 1992, No. 37, pp. 18 – 19.
Endersby NM (1994). Insecticide resistance in cabbage moth. Victorian Vegetable Grower, No. 2, February, 1994, p. 1.
Endersby NM (1994). Further reports of insecticide resistance in cabbage moth. Victorian Vegetable Grower April 1994, pp. 4 – 5.
Endersby NM and Ridland PM (1995). Insecticide resistance in diamondback moths. A challenge for brassica growers. Good Fruit & Vegetables Vol.5 No. 9 (February 1995): 22-23.
Endersby NM and Ridland PM (1998). Diamondback moth (DBM) – What’s going on in the 1998/ 99 season? Access to Asian Vegetables Newsletter, Issue 13 (October 1998).
Endersby NM (1998). 1998/99 Diamondback moth insecticide resistance management strategy. Access to Asian Vegetables Newsletter, Issue 15 (December 1998).
Endersby NM (1999). Start monitoring for diamondback moth. Southern Farmer (p. 4 October 1998 – special Southern Farmer lift-out)
Endersby NM (2001). Dealing with diamondback moth. Southern Farmer, May 2001, p.22
Endersby NM (2001). Scouting for diamondback moth in brassicas. Vegetable Matters – NRE’s news for the Victorian vegetable industries Issue No. 1, June 2001.
Endersby NM (2003). Studying moth movement and insecticide resistance using molecular markers. Brassica IPM National Newsletter Issue 3, July 2003, pp. 2-3.
Endersby NM, McKechnie SW, Ridland PM and Weeks AR (2004). Australia-wide movement of diamondback moth studied using genetic markers. Brassica IPM National Newsletter Issue 5, September 2004, pp.2-3.
Endersby NM (2006) Genetic structure and insecticide resistance in Australian DBM. Brassica IPM National Newsletter Issue 9, August 2006, pp. 3-4.
2016 Invitrogen Science Hero (Behind the scenes category)
2005 Department of Primary Industries Daniel McAlpine Outstanding Achievement Award (Member of Sampling Team)
Working with the PEAR group for many years now, I have both witnessed and participated in its incredible evolution. My work debut involved the miniscule but “mighty in damage” grapevine pest phylloxera and its plant hosts and I quickly learnt to adapt to large scale and often complicated (damaged insects and fibrous roots) genotyping. I also managed the Molecular lab for a short time and was instrumental in the development and implementation of training (equipment and procedural) and EHS practises.
It is fitting that after I became a mother. I returned to the group to work on “the Mother of all dengue vectors”, Aedes aegypti. Here I worked closely with an incredible and inspiring mentor, Nancy Endersby-Harshman, to develop and test genetic markers in Aedes aegypti but also Aedes notoscriptus and Aedes albopictus. Research applications for these markers included population genetics, phylogeography, phenotypic trait association and conversion to new technology platforms (for example, from the P33 radioisotope to the fluorescence based system for microsatellite markers).
Then an endosymbiotic bacterium called Wolbachia exploded into my working life and it was both incredibly promising in terms of its ability to block the dengue virus (and later zika virus) and complex because it was a “novel” vector control strategy with the option of multiple Wolbachia strains whose dengue blocking and host effects (negative and positive) varied. PEARG first focused on the Wolbachia strain wMel and a dedicated period of colony experiments, field releases, molecular testing and post-release monitoring followed providing us with a good understanding of its role in disease suppression. The PEARG captaincy by Professor Ary Hoffmann however soon added other mosquito hosts and Wolbachia strains into the mix and fine-tuned our research goals and approaches to better understand the genetic mechanisms both driving and confounding the spread of Wolbachia in natural populations.
My contributions to this “Wolbachia era” include screening of thousands of Aedes samples for Wolbachia infection status with established “high throughput” light cycler assays but also developing and optimising new light cycler assays including those that can detect very low level and multiple Wolbachia infections in mosquitoes and fruit flies. But by far the most rewarding experiences have been sharing these methodologies with visiting collaborators from China, Indonesia and Sri Lanka and my visit to the wonderful Institute of Medical Research (IMR) in Kuala Lumpur, Malaysia, where I helped adapt our procedures to their working environment. The importance of this IMR collaboration was further highlighted by colleagues who had been infected by dengue three and four times. A job that has the potential to help the lives of many people is something rare and special as is working for Ary, who believes in sharing and advancing the technology and resources that he and his team have developed to fight dengue where it is truly needed.
Recently I hopped over to grasshoppers: So far I have tested mitochondrial and nuclear markers on the parthenogenetic species Warramaba virgo (comprised of two distinct phylads, Standard and Boulder-Xanthus) and maintained a colony of and performed physiological trait measurements on Vandiemenella (another genus of Australian grasshoppers that is made up of different chromosome races). I am now learning about the fascinating Keyacris scurra grasshopper and its conservation plight that restricts it to a handful of regional graveyards. And I am also exploring potential next generation sequencing technologies that can be used to characterise the genetic diversity and evolutionary histories of matchstick grasshoppers (family Morabidae, comprised of over 300 species that do not occur outside Australia) and help identify threats to their future (for example, climate change) and practises that can reduce those threats (for example, translocations of individuals to threatened populations).
Keyacris scurra walks into a Melbourne Uni lab and the scientist says “Why the long face?” and she answers “Because I am at grave risk of extinction”.
*Photo credit: Michael Kearney.
*Molecular training of overseas visitors from Iran and China (mosquito project).
*Development of nuclear SNP markers to detect clonal variation in the parthenogenetic grasshopper Warramaba virgo.
*Ongoing colony maintenance and physiological trait measurements (such as feeding rate) in Vandiemenella grasshoppers.
*Exploring suitable next generation sequencing technologies for understanding the evolutionary histories of and current threats to Morabidae grasshoppers.
Umina, P. A., Corrie, A. M., Herbert, K. S., White, V. L., Powell, K. S. and Hoffmann, A. A. (2007) The use of DNA markers for pest management -clonal lineages and population biology of grape phylloxera. Acta. Hortic.,733, 183-189.
Endersby, N. M., Hoffmann, A. A., White, V. L., Lowenstein, S., Ritchie, S., Johnson, P. H., Rapley, L. P., Ryan, P. A., Nam, V. S., Yen, N. T., Kittiyapong, P. and Weeks, A. R. (2009) Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and Exon-Primed Intron-Crossing markers suggests feasibility of local control options. Journal of Medical Entomology, 46, 1074-1083.
Endersby, N. M., Hoffmann, A. A., White, V. L., Ritchie, S. A., Johnson, P. H. and Weeks, A. R. (2011) Changes in the genetic structure of Aedes aegypti (Diptera: Culicidae) populations in Queensland, Australia, across two seasons: Implications for potential mosquito releases. Journal of Medical Entomology, 48, 999-1007.
Lee, S. F., White, V. L., Weeks, A. R., Hoffmann, A. A. and Endersby, N. M. (2012) High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans. Appl. Environ. Microbiol. 78(13), 4740-4743.
Endersby, N. M., White, V. L., Chan, J., Hurst, T., Rašić, G., Miller, A. and Hoffmann, A. A. (2013) Evidence of cryptic genetic lineages within Aedes notoscriptus (Skuse). Infection, Genetics and Evolution, 18, 191-201.
Rakimov, A., Ben-Dov, Y., White, V. and Hoffmann, A. A. (2013) Soft scale insects (Hemiptera: Coccoidea: Coccidae) on grapevines in Australia. Australian Journal of Entomology, 52, 371-378.
White, V. L., Endersby, N. M., Chan, J., Hoffmann, A. A and Weeks, A. R. (2014) Developing Exon-primed intron-crossing (EPIC) markers for population genetic studies in three Aedes disease vectors. Insect Science, 22(3), 409-423.
Wuliandari, J. R., Lee, S. F., White, V. L., Tantowijoyo, W., Hoffmann, A. A. and Endersby-Harshman, N. M. (2015) Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from Yogyakarta, Indonesia. Insects, 6, 658-685.
Rasic, G., Endersby-Harshman, N., Tantowijoyo, W., Goundar, A., White, V., Qiong, Y., Filipovic, I., Johnson, P., Hoffmann, A. and Arguni, E. (2015) Aedes aegypti has spatially structured and seasonally stable populations in Yogyakarta, Indonesia. Parasites & Vectors, 8, 610.
Hancock, P. A., White, V. L., Callahan, A. G., Godfray, C. H. J., Hoffmann, A. A. and Ritchie, S. A. (2016) Density-dependent population dynamics in Aedes aegypti slow the spread of wMel Wolbachia. Journal of Applied Ecology, 53(3): 785-793.
Hancock, P. A., White, V. L., Ritchie, S. A., Hoffmann, A. A. and Godfrey, H. C. J. (2016) Predicting Wolbachia invasion dynamics in Aedes aegypti populations using models of density-dependent demographic traits. BMC Biology, 14, 96.
Ross, P. A., Wiwatanaratanabutr, I., Axford, J. K., White, V. L., Endersby-Harshman, N. M. and Hoffmann, A. A. (2016) Wolbachia Infections in Aedes aegypti differ markedly in their response to cyclical heat stress. PLOS Pathogens, 13(1): e1006006.
Melissa Carew, Research Fellow
Melissa’s main interests are understanding species diversity using DNA barcodes and developing DNA-based tools for environmental monitoring, especially in freshwater ecosystems. In the past, her research has largely focused on using DNA approaches to understand life histories, population structure and for species identification of various invertebrate (and some vertebrate) groups. During her PhD, she developed DNA approaches for species identification of Chironomidae (Diptera), well known as a bioindicator group in freshwater environments. She also investigated the bioindicator potential in particular chironomid species by examining their field-based responses to pollution. More recently, her work has broadened to include identifying many species of freshwater macroinvertebrates using DNA barcoding.
- Improved biomonitoring of urban freshwater ecosystems using DNA barcodes -ARC Linkage project with Melbourne Water Corporation (LP150100876)
Jeppe, K. J., Carew, M. E., Pettigrove, V., and Hoffmann, A. A. (2017). Toxicant mixtures in sediment alter gene expression in the cysteine metabolism of Chironomus tepperi. Environmental Toxicology and Chemistry 36, 691-698.
Carew, M., Nichols, S., Batovska, J., St Clair, R., Murphy, N., Blacket, M., and Shackleton, M. (2017). A DNA barcode database of Australia’s freshwater macroinvertebrate fauna. Marine and Freshwater Research. Early online.
Jeppe, K. J., Yang, J., Long, S. M., Carew, M. E., Zhang, X., Pettigrove, V., Hoffmann, A. A., and Piggott, J. (2016). Detecting copper toxicity in sediments: from the subindividual level to the population level. Journal of Applied Ecology. Early online.
Carew, M. E., Metzeling, L., St Clair, R., and Hoffmann, A. A. (2016). Detecting invertebrate species in archived collections using next-generation sequencing. Molecular Ecology Resources. Early online.
Carew, M. E., Hoffmann, A. A. (2015). Delineating closely related species with DNA barcodes for routine biological monitoring. Freshwater Biology 60, 1545-1560.
Jeppe, K. J., Carew, M. E., Long, S. M., Lee, S. F., Pettigrove, V., and Hoffmann, A. A. (2014). Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium. Comparative Biochemistry Physiology C Toxicology and Pharmacology 162, 1-6.
Proulx, I., Martin, J., Carew, M., and Hare, L. (2013). Using various lines of evidence to identify Chironomus species.(Diptera: Chironomidae) in eastern Canadian lakes. Zootaxa 3741, 401-458.
Carew, M. E., Pettigrove, V. J., Metzeling, L., and Hoffmann, A. A. (2013a). Environmental monitoring using next generation sequencing: Rapid identification of macroinvertebrate bioindicator species. Frontiers in Zoology 10, 45.
Carew, M., Gagliardi, B., and Hoffmann, A. (2013b). Mitochondrial DNA suggests a single maternal origin for the widespread triploid parthenogenetic pest species, Paratanytarsus grimmii, but microsatellite variation shows local endemism. Insect Science 20, 345-357.
Qian, Z-Q., Sara Ceccarelli, F., Carew, M. E., Schl√ºns, H., Schlick-Steiner, B. C., and Steiner, F. M. (2011). Characterization of polymorphic microsatellites in the giant bulldog ant, Myrmecia brevinoda and the jumper ant, M. pilosula. Journal of Insect Science 11, 1-8.
Carew, M. E., Miller, A. D., and Hoffmann, A. A. (2011a). Phylogenetic signals and ecotoxicological responses: Potential implications for aquatic biomonitoring. Ecotoxicology 20, 595-606.
Carew, M. E., Marshall, S. E., and Hoffmann, A. A. (2011b). A combination of molecular and morphological approaches resolves species in the taxonomically difficult genus Procladius Skuse (Diptera: Chironomidae) despite high intra-specific morphological variation. Bulletin of Entomological Research 101, 1-15.
O’Brien ML, Pettigrove, V., Carew, M. E., Hoffmann, A. A. (2010). Combining rapid bio assessment and field-based microcosms for identifying impacts in an urban river. Environmental Toxicology and Chemistry 29, 1773-1780.
Marshall S, Pettigrove, V., Carew, M., Hoffmann A (2010). Isolating the impact of sediment toxicity in urban streams. Environmental Pollution 158, 1716-1725.
Coleman R, Pettigrove, V., Raadik T, Hoffmann A, Miller A, Carew M (2010). Microsatellite markers and mtDNA data indicate two distinct groups in dwarf galaxias, Galaxiella pusilla; (Mack) (Pisces: Galaxiidae), a threatened freshwater fish from south-eastern Australia. Conservation Genetics 11, 1911-1928.
Townsend, K. R., Pettigrove, V. J., Carew, M. E., and Hoffmann, A. A. (2009). The effects of sediment quality on benthic macroinvertebrates in the River Murray, Australia. Marine and Freshwater Research 60, 70-82.
Schiffer, M., Umina, P., Carew, M., Hoffmann, A., Rodoni, B., and Miller, A. (2009). The distribution of wheat curl mite (Aceria tosichella) lineages in Australia and their potential to transmit wheat streak mosaic virus. Annals of Applied Biology 155, 371-379.
Carew, M., Schiffer, M., Umina, P., Weeks, A., and Hoffmann A (2009). Molecular markers indicate that the wheat curl mite, Aceria tosichella Keifer, may represent a species complex in Australia. Bulletin of Entomological Research 99, 479-486.
Anson, J., Pettigrove, V., Carew, M. E., and Hoffmann, A. A. (2008). High molecular weight petroleum hydrocarbons derived from different sources affect freshwater benthic macroinvertebrate assemblages. Environmental Toxicology and Chemistry 27, 1077-1083.
van Zweden, J., Carew, M., Henshaw, M., Robson, S., Crozier, R. (2007). Social and genetic structure of a supercolonial weaver ant, Polyrhachis robsoni , with dimorphic queens. Insectes Sociaux 54, 34-41.
Cox, R., Hoffmann, A. A., Pettigrove, V., and Carew ME (2007). A microcosm approach for assessing the response of indigenous chironomids to salinity. Australasian Journal of Ecotoxicology 13, 113-118.
Carew, M. E., Pettigrove, V., Cox, R. L., and Hoffmann, A. A. (2007a). The response of Chironomidae to sediment pollution and other environmental characteristics in urban wetlands. Freshwater Biology 52, 2444-2462.
Carew, M. E., Pettigrove, V., Cox RL, and Hoffmann, A. A. (2007b). DNA identification of urban Tanytarsini chironomids (Diptera: Chironomidae). Journal of the North American Benthological Society 24, 586-599.
Carew, M. E., Pettigrove, V., and Hoffmann, A. A. (2005). The utility of DNA markers in classical taxonomy: Using cytochrome oxidase I markers to differentiate Australian Cladopelma (Diptera : Chironomidae) midges. Annals of the Entomological Society of America 98, 587-594.
Carew, M. E., Goodisman, M. A. D., and Hoffmann, A. A. (2004). Species status and population genetic structure of grapevine eriophyoid mites. Entomologia Experimentalis Et Applicata 111, 87-96.
Schiffer, M., Carew, M. E., and Hoffmann, A. A. (2004). Molecular, morphological and behavioural data reveal the presence of a cryptic species in the widely studied Drosophila serrata species complex. Journal of Evolutionary Biology 17, 430-442.
Thomson, L. J., Rundle, B. J., Carew, M. E., and Hoffmann, A. A. (2003). Identification and characterization of Trichogramma species from south-eastern Australia using the internal transcribed spacer 2 (ITS-2). region of the ribosomal gene complex. Entomologia Experimentalis et Applicata 106, 235-240.
Carew, M. E., Pettigrove, V., and Hoffmann, A. A. (2003). Identifying chironomids (Diptera: Chironomidae). for biological monitoring with PCR-RFLP. Bulletin of Entomological Research 93, 483-490.
Magiafoglou, A., Carew, M. E., and Hoffmann, A. A. (2002). Shifting clinal patterns and microsatellite variation in Drosophila serrata populations: a comparison of populations near the southern border of the species range. Journal of Evolutionary Biology 15, 763-774.
Goodisman, M. A. D., Matthews, R. W., Spradbery, J. P., Carew, M. E., and Crozier, R. H. (2001). Reproduction and recruitment in perennial colonies of the introduced wasp Vespula germanica. Journal of Heredity 92, 346-349.
Crozier, R. H., Kaufmann, B., Carew, M. E., Crozier, Y. C. (1999). Mutability of microsatellites developed for the ant Camponotus consobrinus. Molecular Ecology 8, 271-276.
Crozier, R. H., Oldroyd, B. P., Tay, W. T., Kaufmann, B. E., Johnson, R. N., Carew, M. E., and Jennings, K. M. (1997). Molecular advances in understanding social insect population structure. Electrophoresis 18, 1672-1675.
Carew M. E., Tay W. T., and Crozier R. H. (1997). Polygyny via unrelated queens indicated by mitochondrial DNA variation in the Australian meat ant, Iridomyrmex purpureus. Insectes Sociaux 44, 7-14.
2006 PhD – The University of Melbourne
1996 B.Sc (Hon) La Trobe University
Garry McDonald, Research Fellow
Garry has been a research and extension entomologist with the Victorian DPI for 22 years, working initially on mosquito and ant ecology /management, and subsequently on integrated pest management (IPM) and biological control strategies for a range of pests of horticulture, field crops and pastures. Some focal pests of particular interest included Helicoverpa spp. (native budworm and corn earworm), Leucania and Persectania spp. (armyworm), Agrotis spp. (cutworm), pasture cockchafers, Nysius vinitor (Rutherglen bug) and earth mites.
Since 1996, Garry assumed broader administrative roles as Director, Rutherglen Research Institute, subsequently Research Director of DPIs Landscape Systems Sciences Platform, and more recently Director of Swinburne University’s National Centre for Sustainability. In this capacity, he was a Board Director for the Energy Efficiency Council for two years.
Garry joined PEARG in mid-2012 in a part time capacity to undertake research aimed at developing pest forecasting models for the key pests of the crop establishment phase of Australian grain crops – Halotydeus destructor (redlegged earth mite) and Sminthurus viridis (lucerne flea). The modelling focus on developing predictions of both the timing of outbreaks, and their relative severity. He is also the Executive Officer for the Grains Pest Advisory Committee, a national science advisory group for the Grains Research and Development Corporation.
- National Coordination of Invertebrate Pest Research and Insecticide Resistance Management, and the Grains Pest Advisory Committee
Macfadyen, S., McDonald, G., Hill, M. P., (2016). From species distributions to climate change adaptation: Knowledge gaps in managing invertebrate pests in broad-acre grain crops. Agriculture, Ecosystems & Environment. https://doi.org/10.1016/j.agee.2016.08.029
McDonald, G., Umina, P. A., MacFadyen, S., Mangano, P., and Hoffmann, A. A. (2015). Predicting the timing of first generation egg hatch for the pest redlegged earth mite Halotydeus destructor (Acari: Penthaleidae). Experimental and Applied Acarology 65: 259-276 DOI 10.11007/s10493-014-9876-x
Tom joined PEARG in 2014 to undertake a PhD, and since finishing in 2018 is currently employed as a research fellow. His research considers the invasive arbovirus vectors Aedes aegypti (yellow fever mosquito) and Aedes albopictus (Asian tiger mosquito), with an aim to understand how they disperse through anthropogenic environments. This research informs biocontrol efforts at three key stages: identifying invasion routes and source populations, estimating the potential for spatial spread of disease during arbovirus outbreaks, and designing strategies for the elimination or control of invasive populations.
PEARG has been central to the development of vector control strategies involving the release of mosquitoes infected with the bacterium Wolbachia, which leads to either local population eradication or the ongoing suppression of its vectorial capacity. Tom has been involved in these developments directly, investigating spatial dynamics of the three Wolbachia invasions established among Cairns Aedes aegypti in 2013.
- Genetic structure among Asian Aedes albopictus
- Genetic structure among Aedes mosquitoes in and around the Torres Strait
- Bioinformatics as a tool to trace invasion pathways of invasive species
Local introduction and heterogeneous spatial spread of dengue-suppressing Wolbachia through an urban population of Aedes aegypti
TL Schmidt, NH Barton, G Rašić, AP Turley, BL Montgomery, et al.
PLoS Biology 15 (5), e2001894
Genome-wide SNPs reveal the drivers of gene flow in an urban population of the Asian Tiger Mosquito, Aedes albopictus
TL Schmidt, G Rašić, D Zhang, X Zheng, Z Xi, AA Hoffmann
PLoS neglected tropical diseases 11 (10), e0006009
Fine-scale landscape genomics helps explain the slow spread of Wolbachia through the Aedes aegypti population in Cairns, Australia
TL Schmidt, I Filipović, AA Hoffmann, G Rašić
ASTMH Annual Meeting 2016 – Poster Presenter
Australian Entomology Society Conference 2017 – 10 minute talk
Matthew Binns, Research Assistant
Matthew is currently working at PEARG as an entomologist with a focus on agricultural pest management. His background has been mostly based at the University of New England where, from 2008, he studied insect morphology and species diversity over climatic gradients. From 2012, Matthew shifted focus into agricultural issues where he studied attract-and-kill management strategies for Helicoverpa moths in cotton and diamondback moth in canola.
- Insecticide resistance management in red legged earth mites
- Identifying the common earwig species present in grain production systems and classifying them as pests or beneficials
- Quantifying the impact of beneficials on pest populations in grain production systems
Gregg, P., Del Socorro, A.P., and Binns, M. R. (2016). Non-target impacts of an attract-and-kill formulation based on plant volatiles: responses of some generalist predators. Journal of chemical ecology 42.7, 676-688.
Gregg, P., Del Socorro, A.P., Hawes, A.J., and Binns, M. R. (2016). Developing bisexual attract-and-kill for polyphagous insects: ecological rationale versus pragmatics. Journal of chemical ecology 42.7, 666-675.
Gibb, H., Muscat, D., Binns, M. R., Silvey, C. J., Peters, R. A., Warton D. I., and Andrew, N. R. (2015). Responses of foliage-living spider assemblage composition and traits to a climatic gradient in Themeda grasslands, Austral Ecology 40(3), 225-237.
Brown, A. M. Warton, D. I., Andrew, N. R., Binns, M., Cassis, G., and Gibb, H (2014). The fourth-corner solution – using predictive models to understand how species traits interact with the environment. Methods in Ecology and Evolution 5(4), 344-352.
Yates, M. L., Andrew, N. R., Binns, M., and Gibb, H (2014). Morphological traits: predictable responses to macrohabitats across a 300 km scale, PeerJ 2: e271.
Andrew, N. R., Hill, S. J., Binns, M. R., Bahar, M. H., Ridley, E. V., Jung, M. P., Fyfe, C., Yates, M., and Mohammad Khusro (2013). Assessing insect responses to climate change: What are we testing for? Where should we be heading, PeerJ 1: e11.
2015 Predicting the effect of climate change on community structure and function: an assessment using grassland thrips. Ecostats Conference, Sydney, Australia.
2014 Helicoverpa punctigera: Local and Inland overwintering biology. REFCOM conference, Goondiwindi, Australia.
2012 International Congress of Entomology, Daegu, South Korea.
2011 Ecological Society of Australia Conference, Hobart, Australia.
2010 Ecological Society of Australia Conference, Canberra, Australia.
2008 Importance of climate and plant trait variables in structuring beetle communities on Acacia. The Ecological Society of Australia Conference, Sydney, Australia.
I have a BSc joint honours degree in Biochemistry and Pharmacology from King’s College, University of London and an MSc in Natural Resource Management from the University of Leicester, UK. I obtained my PhD in aquatic ecotoxicology from RMIT-University in 2001 where I investigated the effects of crude oil on octopus and mussels. I spent eight years as a terrestrial ecotoxicologist at the Centre for Ecology and Hydrology at Monks Wood in the UK, investigating the effects of pesticides and industrial contaminants on vertebrates and invertebrates using traditional and novel biochemical techniques. I am currently a Research Fellow at CAPIM and PEARG in the School of BioSciences at the University of Melbourne. I have supervised two PhD students (conferred in 2017), three Masters students and a post-graduate diploma student at the University of Melbourne. I am currently supervising two PhD students within the School of BioSciences.
My main research focus is to develop novel methods (predominantly metabolomics, proteins and enzyme activity) to assess biological effects of environmental stressors (anthropogenic and natural) in aquatic organisms. These methods can be used in biomonitoring programs alongside analytical chemistry techniques and community surveys to provide early warning indications of poor water quality to assist in the management of aquatic ecosystems. I am particularly interested in the interaction between stressors and organisms at the individual level to gain a greater understanding of the mechanisms of action of these stressors. This will allow us to better predict adverse effects of mixtures of stressors in the future. I am currently working on a project to develop novel methods of assessing pollution stress in estuarine environments using metabolomics and DNA metabarcoding in collaboration with Melbourne Water and CSIRO.
Bryant S. Gagliardi, Sara M. Long, Vincent J. Pettigrove, Ary A. Hoffmann (2015). The parthenogenetic cosmopolitan chironomid, Paratanytarsus grimmii, as a new standard test species for ecotoxicology: culturing methodology and sensitivity to aqueous pollutants. Bulletin of Environmental Contamination and Toxicology 95 (3) 350-356
Sara M. Long, Dedreia L. Tull, Katherine J. Jeppe, David P. De Souza, Saravanan Dayalan, Vincent J. Pettigrove, Malcolm J. McConville, Ary A. Hoffmann (2015). A multi-platform metabolomics approach demonstrates changes in energy metabolism and the transsulfuration pathway in Chironomus tepperi following exposure to zinc. Aquatic Toxicology 162, 54-65
Katherine J. Jeppe, Melissa E. Carew, Sara M. Long, Siu F. Lee, Vincent Pettigrove, Ary A. Hoffmann. (2014). Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium. Comparative Biochemistry and Physiology Part C. 162, 1-6.
Claudette Kellar, Kathryn L. Hassell, Sara M. Long, Jackie Myers, Lisa Golding., Gavin Rose, Anupama Kumar, Ary Hoffmann, Vincent Pettigrove (2014). Multiple ecological evidence links adverse biological effects to pesticide and metal contamination in an urban Australian watershed. Journal of Applied Ecology. 51 (2), 426-439.
Oliver A.H. Jones,, Steven Murfitt, Claus Svendsen, Anthony Turk, Hazel Turk, David J. Spurgeon, Lee A. Walker, Richard F. Shore, Sara M. Long, Julian L. Griffin. (2013). Comparisons of metabolic and physiological changes in rats following short term oral dosing with pesticides commonly found in food. Food and Chemical Toxicology. 59: 438-445
Fredrik Reichenberg, Ulrich Gosewinkel Karlson, Orjan Gustafsson, Sara M. Long, Parmely H. Pritchard, Philipp Mayer. (2010). Low accessibility and chemical activity of PAHs restrict bioremediation and risk of exposure in a manufactured gas plant soil. Environmental Pollution 158: 1214-1220 (20)
Sara M. Long, Fredrik Reichenberg, Lindsay J. Lister, Peter K. Hankard, Jo Townsend, Philipp Mayer, Julian Wright, Martin Holmstrup, Claus Svendsen, David J. Spurgeon. (2009). Combined chemical (fluoranthene) and drought effects on Lumbricus rubellus demonstrates the applicability of the independent action model for multiple stressor assessment. Environmental Toxicology and Chemistry 28 (3), 629-636
Spurgeon DJ, Rowland P, Ainsworth G, Rothery P, Long S, Black HIJ. (2008). Geographical and pedological drivers of distribution and risks to soil fauna of seven metals (Cd, Cu, Cr, Ni, Pb, V and Zn) in British soils.Environmental Pollution 153: 2, 273-283
Walker, L.A, Turk, A., Long, S.M., Wienburg, C.L., Best, J., & Shore, R.F. (2008). Second generation anticoagulant rodenticides in tawny owls (Strix aluco) from Great Britain. The Science of the Total Environment 392 93-98
- Heywood, J. Wright, C.L. Wienburg, H.I.J. Black, S.M. Long, D. Osborn and D.J. Spurgeon. (2006) Factors Influencing the National Distribution of Polycyclic Aromatic Hydrocarbons and Polychlorinated Biphenyls in British Soils. Environmental Science and Technology 40 7629-7635
SM Long, A. Dawson and RF Shore (2006). A comparison of the effects of single and repeated exposure to an organophosphate insecticide on acetylcholinesterase activity in mammals. Environmental Toxicology and Chemistry 25 (7) 1857-1863
Brown, P.J., Long, S.M., Spurgeon, D.J., Svendsen, C. and Hankard, P. (2004) Toxicological and biochemical responses of the earthworm Lumbricus rubellus to pyrene, a non-carcinogenic polycyclic aromatic hydrocarbon. Chemosphere 57 (11) 1675-1681
SM Long, KJ Ryder and DA Holdway (2003). The Use of Respiratory Enzymes as Biomarkers of Petroleum Hydrocarbon Exposure in Mytilus edulis planulatus. Ecotoxicology and Environmental Safety 55 (3), 261-270
SM Long and DA Holdway (2002). Acute Toxicity of crude and dispersed crude oil to Octopus pallidus (Hoyle, 1885) hatchlings. Water Research 36 (11), 2769-2776.
(presented at national and international conferences, University of Melbourne departmental seminars, stakeholder meetings and research summits; a selection of the most recent presentations is listed below)
Sara M. Long, Dedreia L. Tull, David P. de Souza, Konstantinos A. Kouremenos, Saravanan Dayalan, Jarrad K. Baker, Kathryn L. Hassell, Malcolm J. McConville, Vincent J. Pettigrove, Marthe Monique Gagnon. Environmental metabolomics provides insights into physiological responses of Southern Sand Flathead in Port Phillip Bay. Platform presentation in Special Session on Environmental Stress and its effects on fish at Australian Society of Fish Biology, 21st-24th July 2017, Albany, Australia.
Allyson O’Brien, Sara Long, Anthony Chariton, Mick Keough, Rhys Coleman, Dedreia Tull, Malcolm McConville. Using metabarcoding and metabolomics to measure marine community structure and function. Platform presentation at Australian Marine Science Conference 2017, 2nd-6th July, Darwin, Australia.
Sara M. Long, Georgia M. Sinclair, Allyson L. O’Brien, David P. De Souza, Konstantinos A Kouremenos, Saravanan Dayalan, Ary A. Hoffmann, Michael J. Keough, Malcolm J. McConville, Dedreia L. Tull. Can metabolomics approaches be used to determine the impact of pollution in estuarine environments? Platform presentation at Metabolomics Society conference 26th-29th June 2017, Brisbane, Australia.
Georgia M. Sinclair, Allyson L. O’Brien, Mick Keough, David P. De Souza, Saravanan Dayalan, Dedreia L. Tull and Sara M. Long. Using Metabolomics to Assess the Early Effects of Zinc and Boscalid On Estuarine Polychaete. Platform presentation at Metabolomics Society conference 26th-29th June 2017, Brisbane, Australia.
Dedreia L. Tull, Sara M. Long, David P. de Souza, Konstantinos A. Kouremenos, Jarrad K. Baker, Kathryn L. Hassell, Malcolm J. McConville, Vincent J. Pettigrove, Marthe Monique Gagnon. Environmental metabolomics provides insights into physiological responses of Southern Sand Flathead in Port Phillip Bay. Poster presentation at Metabolomics Society conference 26th-29th June 2017, Brisbane, Australia.
Bryant Gagliardi, Sara Long, Vincent Pettigrove, Ary Hoffmann. Chironomid deformities: are they actually induced by aquatic pollution? Poster presentation at SETAC Europe conference, 7th – 11th May 2017, Brussels, Belgium.
Sara Long and Allyson O’Brien. Developing new tools for monitoring estuarine pollution. Invited presentation at Melbourne Water Waterways Research Stakeholder Forum, December 2nd 2016.
Sara M Long, Georgia M. Sinclair, Allyson L. O’Brien, Dedreia L. Tull, David P. De Souza, Konstantinos A Kouremenos, Saravanan Dayalan, Ary A. Hoffmann, Malcolm J. McConville. Multi-species metabolomics to enable the design of cost-effective biomonitoring programs for determining the impacts of pollution in estuarine environments. Platform presentation at SETAC Europe/UK Environmental Omics Synthesis Centre symposium on environmental and (eco)toxicological Omics and Epigenetics, 12th-15th Sept 2016, Ghent, Belgium.
University of Melbourne/University of Birmingham Travel award 2016
Inaugural SETAC-Au Travel Fellowship 2016
Centre for Ecology and Hydrology New Blood Fellow 2001-2004
I majored in Zoology and Genetics with Honours at Latrobe University, Bundoora and the University of Melbourne. During my honours year in 2009, I developed skills in Drosophila melanogaster DNA sequence analysis and cloning techniques. My professional career began in 2010 as a Research Officer within the PEARG. My skills have expanded since 2010 to include insect husbandry (Aedes aegypti & Aedes notoscriptus), method design and fitness experimentation upon Aedes aegypti, the primary vector of the Dengue virus. The research aims to inform population dynamics models which predict the spread of released mosquitoes artificially infected with an intracellular bacterium, called Wolbachia pipientis, the goal being arbovirus transmission cycle disruption between humans. Other research has involved species distribution modelling upon Aedes albopictus, field work in Cairns and abroad, respirometry, elucidating the transmission cycle of Mycobacterium ulcerans and thermocycler heat stress assays.
NHMRC Dengue Program
Molecular Basis of Adaptive Shift in Body Size in Drosophila melanogaster: Functional and Sequence Analyses of the Dca Gene
Siu F Lee, Ying Chen, Aiden K Varan, Choon W Wee, Ary A Hoffmann
The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations
T Walker, P.H. Johnson, L.A. Moreira, I Iturbe-Ormaetxe, AA Hoffmann
Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission
A.A. Hoffmann, B.L. Montgomery, J Popovici, I Iturbe-Ormaetxe, S L O’Neill
Predicting the spread of Aedes albopictus in Australia under current and future climates: Multiple approaches and datasets to incorporate potential evolutionary divergence
Matthew P. Hill, Jason K. Axford, Ary A. Hoffmann
Assessing quality of life-shortening Wolbachia-infected Aedes aegypti mosquitoes in the field based on capture rates and morphometric assessments
Heng Lin Yeap, Jason K Axford, Jean Popovici, Nancy M Endersby, Ary A Hoffmann
Stability of the wMel Wolbachia Infection following Invasion into Aedes aegypti Populations
Ary A Hoffmann, Inaki Iturbe-Ormaetxe, Ashley G Callahan, Ben L Phillips, Scott L O’Neill
Fitness of wAlbB Wolbachia Infection in Aedes aegypti: Parameter Estimates in an Outcrossed Background and Potential for Population Invasion
Jason K Axford, Perran A Ross, Heng Lin Yeap, Ashley G Callahan, Ary A Hoffmann
Novel applications of thermocyclers for phenotyping invertebrate thermal responses
Jacinta D. Kong, Jason K. Axford, Ary A. Hoffmann, Michael R. Kearney
Wolbachia infections in Aedes aegypti differ markedly in their response to cyclical heat stress
Perran Ross, Itsanun Wiwatanaratanabutr, Jason Axford, Vanessa White, Ary Hoffmann
Mycobacterium ulcerans low infectious dose and mechanical transmission support insect bites and puncturing injuries in the spread of Buruli ulcer
John R. Wallace, Kirstie M. Mangas, Jessica L. Porter, Renee Marcsisin, Timothy P. Stinear
Maintaining Aedes aegypti mosquitoes infected with Wolbachia
Perran A. Ross, Jason K. Axford, Kelly M. Richardson, Nancy M. Endersby-Harshman, Ary A. Hoffmann
Ary keeps me busy managing the lab’s business affairs, occasionally I find time to do a spot of research. I came to science a bit late in the game after several incarnations (audio engineer, archivist, landscape gardener, removalist, ward clerk, night clerk, risk assessor etc.). I completed a bachelor of biological sciences at LaTrobe in 2013, jumped ship to complete an honours year at Melbourne and managed to pull off a first class result under Ary’s supervision. I spent a couple years as a research assistant doing a mix of agricultural consulting work on the road and in the lab. I have a diverse repertoire of skills from bioinformatics to field ecology, lab assays and many points in between. Jack of all trades, master of some. Most of my functions these days are focused on running the labs business affairs, a complex and interesting area to say the least.
Bell, N., Griffin, P.C., Hoffmann, A.A. and Miller, A.D., 2018. Spatial patterns of genetic diversity among Australian alpine flora communities revealed by comparative phylogenomics. Journal of Biogeography, 45(1), pp.177-189.
Kelly has an interest in disease and the roles animals and the environment play in disease transmission. She explored this as an honours student with the PEARG Mosquito Group in early 2008 where she investigated the thermal responses of larval mosquitoes and the influence of climatic changes on their microclimates and potential range in Australia. Since then, she has spent over seven years working with PEARG on a range of projects including the fitness of Aedes aegypti mosquitoes with Wolbachia, the resistance of various wheat lines to the wheat curl mite Aceria tosichella and the wheat streak mosaic and high plains viruses of mites and wheat plants. Since October 2013, Kelly has worked on detecting and characterising Wolbachia in a diverse range of Drosophila species from around Australia. She has detected a number of interesting strains including some male-killers!
Detecting and characterising Wolbachia in a diverse range of Drosophila species
Richardson, K. M., Hoffmann, A. A., Johnson, P., Ritchie, S., Kearney, M. (2011), Thermal sensitivity of Aedes aegypti from Australia: empirical data and prediction of effects on distribution. Journal of Medical Entomology, 48:914-923
Yeap, H. L., Mee P., Walker, T., Weeks, A. R., O’Neill, S. L., Johnson, P., Ritchie, S. A., Richardson, K. M., Doig, C., Endersby, N. M., Hoffmann, A. A. (2011), Dynamics of the “Popcorn” Wolbachia infection in outbred Aedes aegypti informs prospects for mosquito vector control. Genetics, 187:583-595
Richardson, K.M., Hoffmann, A. A., Johnson, P., Ritchie, S.R. and Kearney, M.R. (2012), A replicated comparison of breeding-container suitability for the dengue vector Aedes aegypti in tropical and temperate Australia. Austral Ecology, 38:2019-229
Richardson, K., Miller, A.D., Hoffmann, A.A., Larkin, P. (2014), ‘Potential new sources of wheat curl mite resistance in wheat to prevent the spread of yield-reducing pathogens. Experimental and Applied Acarology, 64:1-19
Richardson, K.M., Schiffer, M., Griffin, P.C., Lee, S.F., Hoffmann, A.A. (2016), Tropical Drosophila pandora carry Wolbachia infections causing cytoplasmic incompatibility or male killing. Evolution, 70:1791-1802
Ross, P.A., Axford, J.K., Richardson, K.M., Endersby-Harshman, N.M., Hoffmann, A.A. (2017). Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia’. Journal of Visualized Experiments, 126:e56124
Turelli, M., Cooper, B.S., Richardson, K.M., Ginsberg, P.S., Peckenpaugh, B., Antelope, C.X., Kim, K.J., May, M.R., Abrieux, A., Wilson, D.A., Bronski, M.J., Moore, B.R., Gao, J., Eisen, M.B., Chiu, J.C., Conner, W.R., Hoffmann, A.A. (in press), Rapid global spread of wRi-like Wolbachia across multiple Drosophila. Current Biology
Australian and New Zealand Society of Comparative Physiology and Biochemistry – Sydney, 2008
2008 B.Sc (Hon) – The University of Melbourne
2008 Diploma of Arts (Environmental Studies) – The University of Melbourne
Best presentation by an Honours student – Conference of the Australian and New Zealand Society of Comparative Physiology and Biochemistry, 2008
Yang is currently working at PEARG as a population geneticist with a focus on insecticide resistance management in red legged earth mites. In the past, her research has largely focused on using techniques of molecular biology and biochemistry to understand role of ceramide-metabolism enzymes in insect development and longevity. During her PhD in Zhejiang University, China, she reported the cloning, biochemical characterization, and functional analysis of Drosophila alkaline ceramidase (Dacer), suggesting that Dacer plays an important role in the Drosophila development and longevity by controlling the metabolism of ceramides. She then worked as a research fellow in Jiangsu academy of agricultural sciences, China, characterizing ceramidase and sphingomyelinase in Chilo suppressalis. She was also involved in transcriptome analysis of Scirpophaga incertulas with focus on bet-hedging genes. From 2014, Yang shifted focus into population genetics where she explored fine-scale processes of gene flow and dispersal in resistant red legged earth mites. This knowledge can be applied to better understand the routes resistant individuals have dispersed along, that may be important in reducing the risk of resistance spreading further.
Insecticide resistance management (population genetics) in red legged earth mites
NHMRC Dengue Program
Refereed Journals (first author)
Yang Q, Sun N, Zheng M, Luo CC, Luo GH, Fang JC*，Construction of genomic ddRADseq libraries of Chilo suppressalis and Scirpophaga incertulas (Lepidoptera: Pyralidae). Nature Enemies of Insects, 2016 (6): 1114-1120.
Yang Q, Li XW, Lin XW, Zhou Y, Yuan JQ, Wang HD, Chen JA, Mao CG , Zhu ZR*, Characterization of free endogenous sphingoid bases in the golden apple snail Pomacea canaliculata: involvement in snail development and nutrient limitation. Invertebrate Reproduction & Development, 2013, 57(4): 287 – 292.
Yang Q, Zhou Y, Han GJ, Fang JC*. Isolation, identification and active ingredient analysis of two pathogenic bacterial strains from Scirpophaga incertulas (Lepidoptera: Pyralidae). Jiangsu Journal of Agricultural Science. 2012, 28(6): 1267-71.
Yang Q, Gong ZJ, Zhou Y, Yuan JQ, Cheng JA, Tian L, Li S, Lin XD, Xu RJ, Zhu ZR* Mao CG*, Role of Drosophila alkaline ceramidase (Dacer) in Drosophila development and longevity. Cellular and Molecular Life Sciences, 2010, 67(9): 1477-1490.
Refereed Journals – co-author
Zhang YL, Han YC, Liu BS, Yang Q, Guo HF, Liu ZW, Wang LH, Fang JC*, Resistance monitoring and cross-resistance role of CYP6CW1 between buprofezin and pymetrozine in field populations of Laodelphax striatellus (Fallen). Scientific Reports, 2017, 7: 14639.
Zhou Y, Lin XW, Begum MA, Zhang CH, Shi XX, Jiao WJ, Zhang YR, Yuan JQ, Li HY, Yang Q, Mao CG, Zhu ZR*, Identification and characterization of Laodelphax striatellus (Insecta: Hemiptera: Delphacidae) neutral sphingomyelinase. Insect Molecular Biology, 2017, 6(4): 392-420.
Luo GH, Yao J, Yang Q, Zhang ZC, Hoffmann AA, Fang JC*, Variability in development of the striped rice borer, Chilo suppressalis (Lepidoptera: Pyralidae), due to instar number and last instar duration. Scientific Reports, 2016, 6: a35231.
Rašic G*, Endersby-Harshman NM, Tantowijoyo W, Goundar A, White VW, Yang Q, Filipovid I, Johnson P, Hoffmann AA, Arguni E, Aedes aegypti has spatially structured and seasonally stable populations in Yogyakarta, Indonesia. Parasites and Vectors, 2015, 8: 610.
Zhou Y, Yang Q, Han GJ, Fang JC*, Isolation of genomic microsatellite molecular markers with enrichment of suppression-PCR in Scirpophaga incertulas (Lepidoptera: Pyralidae). Jiangsu Journal of Agricultural Science, 2013, 29(5): 999-1004.
Zhang YL, Guo HF, Yang Q, Li S, Wang LH, Zhang GF, and Fang JC*. Overexpression of a P450 gene (CYP6CW1) in buprofezin-resistant Laodelphax striatellus (Fallén). Pesticide Biochemistry and Physiology, 2012, 104 (3): 277-282.
Zhou Y, Lin XW, Yang Q, Zhang YR, YuanJQ, Ling XD, Xu RJ, Cheng JA, MaoCG*, Zhu ZR*. Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum. Biochimie, 2011, 93 (7): 1124-1131.